摘要
过氧化氢酶是影响微生物生命活动的关键酶,为揭示混合污染物对垃圾渗滤液中微生物过氧化氢酶活性的影响及其相互作用关系,选取垃圾渗滤液中常见的钙镁离子作为污染物,利用直接均分射线法设计3种浓度配比的钙镁离子混合物,钙离子占比分别为0.27、0.52、0.77;然后,根据抑制效应的变化分析钙镁离子对过氧化氢酶活性的抑制作用,并利用等效线图法分析钙镁离子对垃圾渗滤液中过氧化氢酶活性作用的相互关系.结果表明:①钙镁离子及其混合物对过氧化氢酶活性的抑制效应具有相同的规律,在同一周期下抑制效应随着钙镁离子浓度的增加而增大,在同一浓度下抑制效应随着周期的增加而增大.②当钙离子占比为0.27时,钙镁离子混合物对过氧化氢酶活性作用的相互关系主要表现为协同作用;当钙离子占比为0.52、0.77时,钙镁离子混合物对过氧化氢酶活性作用的相互关系主要表现为拮抗作用.研究显示,随着钙离子占比的增加,钙镁离子混合物对过氧化氢酶活性作用的相互关系由以协同作用为主变为以拮抗作用为主.
Catalase is an important enzyme that affects the activities of microorganisms.To reveal the effect of mixed pollutants on microbial catalase activity and their interaction during the treatment of landfill leachate,this study investigated the effect of calcium and magnesium as pollutants.Three concentration ratios of calcium and magnesium were designed by the direct equipartition ray method,and the ratios were 0.27,0.52 and 0.77.Then the inhibition effect of calcium and magnesium on catalase activity was analyzed according to the change of inhibition rate.Finally,the correlation of calcium and magnesium ions to catalase activity was studied using the isobolograms.The results showed that:(1)There were obvious increase in the inhibitory effects of single pollutant and mixed pollutants on microbial catalase activity with the increase of reaction time and the pollutant concentration.(2)When the proportion of calcium ions was 0.27,the interaction between calcium ions and magnesium ions was mainly synergistic.However,when the proportion of calcium ions was 0.52 and 0.77,the interaction relationship between calcium ions and magnesium ions was mainly antagonistic.This study shows that with the increase of the proportion of calcium ions,the interaction between calcium ions and magnesium ions changes from synergistic effect to antagonistic effect.
作者
张华
庄雪晴
黄健
贾旋
奚姗姗
王金花
ZHANG Hua;ZHUANG Xueqing;HUANG Jian;JIA Xuan;XI Shanshan;WANG Jinhua(School of Environment and Energy Engineering,Anhui Jianzhu University,Hefei 230601,China;Key Laboratory of Water Pollution Control and Wastewater Reuse of Anhui Province,Hefei 230601,China)
出处
《环境科学研究》
CAS
CSCD
北大核心
2022年第1期173-180,共8页
Research of Environmental Sciences
基金
安徽省高校省级自然科学研究项目(No.KJ2019ZD52,KJ2020A0466)
国家重点研发计划项目(No.2019YFC0408504)。
