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藏药乌奴龙胆的黄酮含量测定研究 被引量:3

Study on the content determination of flavonoids in Tibetan medicine Gentiana urnula
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摘要 目的:分别测定乌奴龙胆不同批次、不同部位的总黄酮含量,并比较所含总黄酮含量。方法:将3批乌奴龙胆及第3批乌奴龙胆的茎、叶、花分别粉碎,用超声提取,得提取液,采用紫外-可见分光光度法,以芦丁为对照品测定总黄酮含量。结果:3批乌奴龙胆的总黄酮含量分别为33.33 mg·g^(-1)、32.48 mg·g^(-1)、40.23 mg·g^(-1);第3批乌奴龙胆茎、叶、花中总黄酮含量为32.34 mg·g^(-1)、57.74 mg·g^(-1)、72.15 mg·g^(-1)。结论:不同批次的乌奴龙胆总黄酮含量会有所差异;乌奴龙胆茎、叶、花中都含有黄酮类成分,花中的含量最多,茎中的含量最少。 Objective:The content of total flavonoids in different batches and parts of Gentiana urnula were determined,and the differences in total flavonoids content were compared.Methods:The stems,leaves and flowers of the 3 batches of Gentiana urnula and the third batch of Gentiana urnula were crushed separately,and then extracted with ultrasound to obtain the relevant extracts,which were determined by ultraviolet-visible spectrophotometry with rutin as the reference substance.Total flavonoid content.Result:The total flavonoids of the three batches of Gentiana urnula were 33.33 mg·g^(-1),32.48 mg·g^(-1),and 40.23 mg·g^(-1);The total flavonoids in the stems,leaves,and flowers of the third batch of Gentiana urnula were32.34 mg·g^(-1),57.74 mg·g^(-1),72.15 mg·g^(-1).Conclusion:The content of total flavonoids in different batches of Gentiana urnula varies;the stems,leaves and flowers of Gentiana urnula all contain flavonoids.Among them,the content in the flower is the most,and the content in the stem is the least.
作者 郑海婷 严志宏 顿珠次仁 ZHENG Hai-ting;YAN Zhi-hong;DUNZhu Ciren(Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China;Tibetan Traditional Medical College,IJiasa 850000,China)
出处 《中国民族医药杂志》 2022年第2期42-44,共3页 Journal of Medicine and Pharmacy of Chinese Minorities
基金 2018年西藏自治区自然科学基金项目——藏药乌奴龙胆质量多指标评价及指纹图谱研究(编号:XZ2018 ZR G-128)。
关键词 乌奴龙胆 紫外-可见分光光度法 黄酮 含量测定 Gentiana urnula Ultraviolet-visible spectrophotometry Flavonoids Assay
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