摘要
目的:采用响应面法优化固载乳酸菌发酵中药槐角发酵体系的最佳条件,为槐角药用资源的进一步开发利用提供参考。方法:以发酵体系中染料木素含量为指标,通过中心组合试验原理设计单因素试验,并结合响应面法考察乳酸菌发酵后pH值、温度和液料比对发酵体系中总黄酮和染料木素含量的影响。结果:最佳发酵条件为pH 5.0,时间36 h,液料比25∶1(mL/g),优化后槐角发酵体系中总黄酮的含量达到(122.00±0.56)mg/g,染料木素含量(18.93±0.47)mg/g,为原药材的2.90倍。结论:采用单因素优化结合响应面分析对固载乳酸菌发酵中药槐角体系条件进行优化,发酵后染料木素和总黄酮的含量极大提高,优化后的新工艺可在实际应用中推广,为中药槐角的开发利用奠定了基础,提供了新途径。
Objective:To optimize the fermentation conditions of Fructus Sophorae by immobilized lactic acid bacteria(LAB)by using response surface methodology(RSM),thus to provide reference for the further development of medicinal resource of Fructus Sophorae.Methods:The content of genistein in the fermentation system was taken as an index,and a single factor experiment was conducted by central composite design,the effects of pH value,time and liquid-solid ratio on the contents of total flavonoids and genistein in the fermentation system were investigated by response surface methodology.Results:The optimum processing conditions were as follows,pH was 5.0,time duration was 36 h,liquid-solid ratio was 25∶1(mL/g),the content of total flavonoids was(122.00±0.56)mg/g,and the optimum content of genistein was(18.93±0.47)mg/g,which was 2.90 times to the untreated sample.Conclusion:The single factor and response surface method were used to optimize the fermentation conditions of Fructus Sophorae with immobilized LAB.The contents of genistein and total flavonoids were greatly increased in the optimal fermentation condition.The optimized process can be applied in practice,which lays a foundation and provides a new way for the development and utilization of Fructus Sophorae.
作者
金爽
白雪
许雯惠
陈威沛
吕晨
程玉鹏
崔喆
匡海学
JIN Shuang;BAI Xue;XU Wenhui;CHEN Weipei;LYU Chen;CHENG Yupeng;CUI Zhe;KUANG Haixue(Key Laboratory of Basic and Application Research of Beiyao, Ministry of Education, Heilongjiang University of Chinese Medicine, Harbin 150040, China)
出处
《中医药学报》
CAS
2022年第1期47-51,共5页
Acta Chinese Medicine and Pharmacology
基金
黑龙江省自然科学基金面上项目(H2018053)
中国博士后基金特别资助项目(2018T110321)
中国博士后基金面上资助项目(2016M591567)
黑龙江省博士后基金资助项目(LBH-Z15206)
黑龙江中医药大学优秀青年教师支持计划项目(2018RCQ10)
黑龙江中医药大学大学生科技创新项目(2021-2)
黑龙江中医药大学大学生创新创业训练计划项目(X202110228002)。
关键词
固载
乳酸菌
槐角
总黄酮
Immobilization
Lactic acid bacteria
Fructus Sophorae
Total flavonoids
