摘要
利用裂褶菌(Schizophyllum commune)DS1分泌的β-葡萄糖苷酶对槐角中异黄酮进行酶法转化,制备染料木素,采用高效液相色谱法检测转化前后染料木素的含量.结果表明β-葡萄糖苷酶转化槐角异黄酮的最佳转化条件是:pH=4.6,反应温度37℃,反应时间6h,转化后的染料木素量比转化前的提高了18倍多;分析高效液相色谱图,研究认为β-葡萄糖苷酶转化槐角异黄酮过程为三糖苷转化成双糖苷,再从双糖苷转化成单糖苷,最终转化成甙元,如染料木素.
β-glucosidase originated from Schizophyllum commune DS1 transforms the isoflavones of Sophora japonica L. for preparation of genistein, and high performance liquid chromatography （HPLC） method was used to analyze the isoflavones. The optimal transformation＇condition was as following： pH：4.6, 37 ℃ as reaction temperature, 6 h as the reaction time. Under the optimal con- dition, the content of genistein was more than 18 times as much as the content before transformation by the β-glucosidase. Comparison the HPLC, the biotransformation pathway deduction was that the isoflavone triglycoside was firstly hydrolyzed to isoflavone diglycoside, then to isoflavone glycoside, at last to isoflavone aglycone, such as genistein.
出处
《华中科技大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2010年第8期125-128,共4页
Journal of Huazhong University of Science and Technology(Natural Science Edition)
关键词
高效液相色谱
优化
酶水解
裂褶菌
槐角
异黄酮
染料木素
high performance liquid chromatography
optimization
enzymatic hydrolysis
Schizophyllum commune
Sophorajaponica L.
isoflavone
genistein
