摘要
[目的]分析腺苷酸环化酶1(ADCY1)和胰凝乳蛋白酶(CYM)基因单核苷酸多态性(SNPs)与山羊产羔性状的关联。[方法]通过MassARRAY SNP分型技术对ADCY1和CYM基因SNPs位点进行分型,检测其在云上黑山羊(n=544)、济宁青山羊(n=133)和辽宁绒山羊(n=91)3个群体中的遗传学特征,并将其多态性与产羔性能(产羔数、初生窝重、断奶窝重)进行关联分析。[结果]群体遗传学结果表明,ADCY1基因4个突变在云上黑山羊、济宁青山羊、辽宁绒山羊中均为低度多态(PIC≤0.25);CYM基因g.87624254G>A和g.87627082G>A 2个位点在3种山羊中均为中度多态(0.25<PIC<0.50);卡方检验显示,除ADCY1 g.43996583C>T在辽宁绒山羊中处于Hardy-Weinberg不平衡状态(P<0.05),ADCY1基因其余位点和CYM基因g.87624269 G>A、g.87627082G>A 2个位点在3个群体中均处于Hardy-Weinberg平衡状态(P>0.05)。关联分析结果表明,在云上黑山羊中ADCY1基因g.43979904G>A、g.43974472G>A,CYM基因g.87624254G>A位点多态性与产羔数存在显著关联(P<0.05);ADCY1基因g.43996550G>A、g.43996583C>T,CYM基因g.87624254G>A、g.87627082G>A位点多态性与初生窝重存在显著关联(P<0.05)。[结论]ADCY1基因g.43974472G>A和g.43979904G>A位点可作为云上黑山羊产羔数选择的潜在分子标记。ADCY1基因g.43996550G>A、g.43996583C>T位点,CYM基因g.87627082G>A位点可作为云上黑山羊初生窝重选择的潜在分子标记;CYM基因g.87624254G>A位点可作为云上黑山羊窝重选择的潜在分子标记。
[Objective]This study aimed to analyze the association between single nucleotide polymorphisms(SNPs)in the adenylate cyclase 1(ADCY1)and chymotrypsin(CYM)genes and kidding performance in goats.[Method]MassARRAY SNP typing technology was applied to genotype the SNPs of ADCY1 and CYM genes and detect their genetic characteristics in Yunshang black goat(n=544),Jining grey goat(n=133)and Liaoning cashmere goat(n=91).Then the association was analyzed between ADCY1 and CYM and kidding performance(litter size,litter weight at birth,litter weight at weaning)in different goats.[Result]The results of population genetics showed that the 4 mutations of ADCY1 gene were low polymorphisms in Yunshang black goat,Jining grey goat,and Liaoning cashmere goat(PIC≤0.25);CYM gene g.87624254G>A and g.87627082G>A both candidate locus were moderately polymorphic in the three goats(0.25<PIC<0.5).Chi-square test showed that,except ADCY1 g.43996583C>T in Liaoning cashmere goats,which is in Hardy-Weinberg imbalance(P<0.05),the other locus are in Hardy-Weinberg equilibrium(P>0.05);CYM gene g.87624269 two locus G>A and g.87627082G>A were in Hardy-Weinberg equilibrium in the three populations(P>0.05).The results of association analysis showed that in Yunshang black goats,the ADCY1 gene g.43974472G>A,g.43979904G>A,and CYM gene g.87624254G>A polymorphisms were significantly associated with litter size(P<0.05);ADCY1 gene g.43996550G>A,g.43996583C>T,CYM gene g.87624254G>A,g.87627082G>A polymorphisms were significantly associated with the primary litter weight(P<0.05).[Conclusion]In summary,ADCY1 gene g.43974472G>A and g.43979904G>Acan be used as a potential molecular marker for litter size in Yunshang black goats.ADCY1 geneg.43996550G>A and g.43996583C>T locus,CYM gene g.87627082G>A locus can be used as potential molecular markers for litter weight at birth in Yunshang black goats;CYM gene g.87624254G>A locus can be used as potential molecular markers for litter weight selection in Yunshang black goats.
作者
李昆谕
洪琼花
陶林
江炎庭
欧阳依娜
储明星
刘玉芳
LI Kun-yu;HONG Qiong-hua;TAO Lin(College of Life Science and Food Engineering,Hebei University of Engineering,Handan,Hebei 056001;Key Laboratory of Animal Genetics,Breeding and Reproduction of Ministry of Agriculture and Rural Affairs,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193;Yunnan Animal Science and Veterinary Institute,Kunming,Yunnan 650224)
出处
《安徽农业科学》
CAS
2021年第24期141-145,162,共6页
Journal of Anhui Agricultural Sciences
基金
国家肉羊产业技术体系专项(CARS-38)
河北省自然科学基金青年项目(C2019402261)
云南省科技创新人才计划(2018HC017)
云南省万人计划——产业技术领军人才项目
中国农业科学院科技创新工程(CAAS-ZDRW202106和ASTIP-IAS13)。
