摘要
目的制备氧化再生纤维素/壳聚糖复合物(ORC-CTS)止血海绵并研究其性能。方法制备并表征ORC-CTS止血海绵。通过体内止血实验、炎症反应及毒性分析,比较ORC-CTS与壳聚糖、速即纱的体内止血性能及效果。结果ORC-CTS组的出血量明显少于空白对照组、壳聚糖组、速即纱组(均P<0.05),止血时间亦明显短于空白对照组、壳聚糖组、速即纱组(均P<0.05);炎症程度依次为对照组<ORC-CTS<壳聚糖<速即纱组;ORCCTS组血清TNF-α、异前列腺素和vWF与对照组无差异。结论ORC-CTS能显著减少出血量,缩短出血时间。生物体内炎症反应轻,有良好的生物相容性,在生物体内几乎无毒,有望成为一种理想的新型止血材料。
Objective To prepare oxidized regenerated cellulose/chitosan(ORC-CTS)hemostatic sponges,and study its properties.Methods ORC-CTS hemostatic sponges were prepared and characterized.The hemostatic properties and prognostic effects of ORC-CTS,chitosan,and surgicel were compared by hemostasis test,inflammation test,and toxicity test in vivo.Results The blood loss in the ORC-CTS group was less than that in the control group,the chitosan group,and the surgicel group(all P<0.05);the hemostasis time in the ORC-CTS group was shorter than that in the control group,the chitosan group,and the surgicel group(all P<0.05).The degree of inflammation ranked as control group<ORC-CTS group<chitosan group<surgicel group.No significant differences in serum tumor necrosis factor(TNF-α),isoprostanes,and von Willebrand Factor(vWF)were found between the ORC-CTS group and the control group.Conclusion ORC-CTS hemostatic sponges can reduce blood loss,shorten bleeding time,stimulate mild inflammatory reaction,show good biocompatibility and little toxicity,thus may be an ideal new hemostatic material.
作者
黄治曙
周立新
苏继钦
严辉弟
林艳丽
HUANG Zhishu;ZHOU Lixin;SU Jiqin;YAN Huidi;LIN Yanli(Department of Hepatobiliary Surgery,The Second Affiliated Hospital of Xiamen Medical College,Xiamen 361021,Fujian,China;Department of Pathology,The Second Affiliated Hospital of Xiamen Medical College,Xiamen 361021,Fujian,China)
出处
《外科研究与新技术》
2021年第3期167-171,共5页
Surgical Research and New Technique
关键词
氧化再生纤维素
壳聚糖
止血海绵
Oxidized regenerated cellulose
Chitosan
Hemostatic sponge
