摘要
为明确苹果端粒酶特性与组培试管苗繁殖、衰老的关系,本试验选取‘金冠‘’富士’及‘嘎拉’3个苹果品种的不同继代次数、不同苗龄的试管苗叶片及不同的试管材料(试管苗叶片,茎段,愈伤组织),分别用改良TRAP法和实时荧光定量PCR法测定了端粒酶活性和端粒酶逆转录酶基因表达量。研究表明,随继代次数增加,保存多年的苹果试管苗端粒酶活性变化不显著;苹果试管苗在90 d的生长期内,不同苗龄叶片端粒酶活性呈现先升高后降低的趋势;不同试管材料端粒酶活性具有组织特异性,其中‘金冠’愈伤组织端粒酶活性最高,‘嘎拉’叶片的端粒酶活性最高。本研究中端粒酶逆转录酶基因表达量与端粒酶活性的变化规律一致。
In order to understand the telomerase characteristics in relation to the proliferation and aging of the tissue culture plantlets of apple,the leaves,stems,and callus sampled from Malus domestica’Golden Delicious’,Malus domestica’Fuji’,and Malus domestica’Gala’plantlets that subcultured in vitro for years were analyzed for the telomerase activity and the amount of telomerase reverse transcriptase gene expression by using modified TRAP and Quantitative Real-time PCR.The results showed that the telomerase activity in the leaves and stems had no significant difference among the plantlets that subcultured for different times.During a given subculture time,the telomerase activity in leaves showed a tendency of increasing first and then declining along with the leaf aging in a ninety-day period.The telomerase activity varied in different materials,among which Malus domestica’Golden Delicious’callus and Malus domestica’Gala’leaves appeared the highest amount,respectively.The expression of telomerase reverse transcriptase gene was consistent with the change of telomerase activity.
作者
梁晨
刘雅洁
王莉
杜国强
师校欣
Liang Chen;Liu Yajie;Wang Li;Du Guoqiang;Shi Xiaoxin(College of Horticulture,Hebei Agricultural University,Baoding,071000)
出处
《分子植物育种》
CAS
北大核心
2021年第13期4472-4477,共6页
Molecular Plant Breeding
基金
河北省自然科学基金项目(C2018204055)资助。
关键词
苹果
试管苗
继代次数
苗龄
端粒酶活性
端粒酶逆转录酶
Apple
Plantlet in vitro
Subcultured times
Plantlet aging
Telomerase activity
Telomerase reverse transcriptase
