摘要
以欧李3号、4号品种为试材,初步建立了茎尖组织培养的快繁体系。结果表明:欧李对激素反应比较敏感。分化基本培养基采用MS优于B5和W h ite。在MS+0.25mg.L-1BA+0.1mg.L-1IAA的分化培养基上,萌发速度快,成苗率高。增殖培养时,增殖系数随BA浓度增大而提高;欧李3号在MS+0.5mg.L-1BA+0.075mg.L-1NAA的培养基上,增殖系数为5.43;欧李4号在MS+0.4mg.L-1BA+0.05mg.L-1NAA的培养基上,增殖系数为4.56,增殖效果较好。壮苗培养时,欧李3号适宜的培养基为改良MS+0.075mg.L-1NAA,欧李4号适宜的培养基为改良MS+0.05mg.L-1NAA。生根培养时,欧李3号适宜的培养基为1/2MS+0.4mg.L-1NAA;生根率达72.3%,欧李4号适宜的培养基为1/2MS+0.2mg.L-1NAA;生根率达74.6%。移栽采用草炭、田园土、珍珠岩(5∶2∶1),成活率达72.7%。
Rapid propagation of shoot -tip culture system was set up with Ouli3 and 4 as materials. The main results were as follows： Ouli was found sensitive to hormone. Medium MS was better for differentiation than B5 and white. The high rate of bourgeon and survival occurred in Medium MS ＋ 0. 25 mg · L^-1 BA ＋ 0. 1 mg· L^-1 IAA. As for multiplication culture, positive correlation was observed with multiplication coefficient and BA concentration. The coefficients were 5.43 and 4. 56 for Ouli 3 in MS ＋0. 05 mg· L^-1 BA ＋0. 075 mg· L^-1 NAA and for Ouli 4 in MS ＋0. 4mg· L^-1 BA ＋ 0. 05 mg · L^-1 NAA, respectively. The modified medium MS ＋0.75 mg· L^-1 NAA was optimum for healthy seedling culture of Ouli 3, and the best one for Ouli 4 was MS ＋0. 05 mg · L^-1 NAA. 1/2MS ＋0.4mg· L^-1. NAA was the optimum medium for rooting culture of Ouli 3, while for Ouli 4,the optimum one was 1/2MS ＋ 0. 2mg· L^-1 NAA. The rooting rate was 72.3% and 74. 6% respectively. Survival rate was 72.7% when the seedlings were transplanted in peat ＋ garden soil ＋ peadite（5： 2：1 ）.
出处
《沈阳农业大学学报》
CAS
CSCD
北大核心
2006年第4期573-577,共5页
Journal of Shenyang Agricultural University
关键词
欧李
茎尖
组织培养
Cerasus humilis
shoot-tip
tissue culture
