摘要
以多刺绿绒蒿为原材料,采用响应面法分析优化超声辅助提取多刺绿绒蒿总黄酮的工艺。在单因素试验的基础上,以乙醇浓度、超声温度、超声时间为影响因素,总黄酮得率为响应面值,优化超声辅助多刺绿绒蒿总黄酮的提取工艺。结果表明,当乙醇浓度为70%、超声温度为60℃、超声时间为60min时黄酮得率最高为3.21%。该条件下多刺绿绒蒿清除DPPH、ABTS的IC50值分别为27.064、15.473μg/mL。
Using Meconopsis horridula Hook.f.&Thomson as the raw material,response surface analysis was used to optimize the ultrasonic-assisted extraction process of total flavonoids in Meconopsis horridulaHook.f.&Thomson.On the basis of single factor experiment,ethanol concentration,ultrasonic temperature,and ultrasonic time were used as influencing factors,and the total flavonoid yield was the response surface value,and the ultrasonic-assisted extraction process of total flavonoids of Meconopsis horridula Hook.f.&Thomson was optimized.The results showed that when the ethanol concentration was 70%,the ultrasonic temperature was 60℃,and the ultrasonic time was 60min,the highest flavonoid yield was 3.21%.Under this condition,the IC50 of Meconopsis horridula Hook.f.&Thomson against DPPH and ABTS were 27.064 and 15.473μg/mL,respectively.
作者
马静
郭阳
张国英
张得钧
MA Jing;GUO Yang;ZHANG Guoying;ZHANG Dejun(College of Eco-Environmental Engineering,Qinghai University,Xining 810016;College of Medical,Qinghai University,Xining 810001)
出处
《中国食品添加剂》
CAS
北大核心
2021年第6期31-38,共8页
China Food Additives
基金
中国科学院青海省人民政府三江源国家公园联合研究专项项目(LHZX-2020-09)。
关键词
多刺绿绒蒿
黄酮
超声波辅助提取
响应面优化
抗氧化
Meconopsis horridula Hook.f.&Thomson
flavonoids
ultrasonic-assisted extraction
response surface optimization
anti-oxidation
