摘要
目的探讨CYP3A4的基因多态性与雷公藤多苷片治疗类风湿关节炎(RA)疗效个体差异的相关性。方法收集58例口服雷公藤多苷片的RA患者,采用SnaPshot法检测患者CYP3A4基因的单核苷酸多态性(SNP),并以Pearson Chi-Square test进行ardy-Weinberg平衡验证和关联性检验,以二分类Logistic回归模型分析CYP3A4基因型与RA患者服用雷公藤多苷片疗效个体差异之间的关系。结果58例患者的基因型达到了Hardy-Weinberg遗传平衡(P>0.05),3种基因型(GG型、GA型、AA型)的有效率差异有统计学意义,当两两比较时,GG型与AA型的有效率差异无统计学意义,而GG型与GA型、GA型与AA型组间的有效率差异有统计学意义。结论CYP3A4基因多态性与雷公藤多苷片治疗RA疗效个体差异有关,AA型患者服用雷公藤多苷片最有效,GA型次之,GG型最差。
Objective To investigate the correlation between CYP3A4 gene polymorphism and individual differences in the therapeutic effect of Tripterygium wilfordii on rheumatoid arthritis(RA).Methods 58 RA patients taking tripterygium glycosides tablets orally were selected and CYP3A4 single nucleotide polymorphism(SNP)were detected by SnaPshotmethod.Hardy-Weinberg equilibrium verification and correlation test were performed using Pearson Chi-Square test.The relationship between CYP3A4 genotype and the therapeutic effect of tripterygium wilfordii in patients with rheumatoid arthritis was analyzed by binary Logistic regression model to see the individual differences between CYP3A4 gene polymorphism and the therapeutic effect of Tripterygium wilfordii on rheumatoid arthritis.Results Genotype to Hardy-Weinberg genetic balance was reached in 58 patients with(P>0.05),the three genotypes(GG,GA,AA)efficient difference was statistically significant;when comparing in pair,there was no statistically significant difference in the effective rate between GG and AA type(P>0.05),and but was statistically significant difference between GA and AA type efficient difference(P<0.05).Conclusion The individual difference in therapeutic effect of tripterygium wilfordii in patients with rheumatoid arthritis was related to SNP polymorphism of CYP3A4 gene.It was most effective to in AA type of patient taking Tripterygium wilfordii,then less in GA type,and lest in GG type.
作者
朱光昭
王翰洲
杨豫正
高扬
白健
赵言鹏
王仁一
王西西
杨征征
王海隆
ZHU Guang-zhao;WANG Han-zhou;YANG Yu-zheng;GAO Yang;BAI Jian;ZHAO Yan-peng;WANG Ren-yi;WANG Xi-xi;YANG Zheng-zheng;WANG Hai-long(Rheumatology Department,Qinghai Hospital of TCM,Xining 810000,China;Rheumatology Department,Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053;Guizhou University of Traditional Chinese Medicine,Guiyang 550025;Rheumatology Department,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700)
出处
《北京中医药》
2021年第4期338-341,共4页
Beijing Journal of Traditional Chinese Medicine
基金
国家自然科学基金面上项目(81673804)
国家人社部留学人员科技活动项目择优项目(优秀类)(31470962)
中国中医科学院基本科研业务费自主选题项目(ZZ0708079)。
