摘要
目的基于网络药理学探讨丹参治疗类风湿关节炎的分子机制。方法利用中药系统药理数据库与分析平台(TCMSP)获取丹参的活性成分及靶点,与GeneCards、OMIM数据库获取的类风湿关节炎疾病基因靶点映射,构建药物-活性成分-疾病靶点网络。DAVID数据库中进行基因本体论(GO)功能富集和京都基因和基因组百科全书(KEGG)通路富集分析。结果从丹参中筛选到56种活性成分,作用靶点基因100个;检索类风湿关节炎疾病靶点基因1640个;取丹参与类风湿关节炎疾病靶点基因交集共得到44个共同靶点,其中13个为关键靶点。获得96条GO生物学过程和25条KEGG信号通路,涉及免疫调节、炎症反应及细胞凋亡等。结论丹参可能通过多成分、多靶点网络来治疗类风湿关节炎。
Objective To explore the potential molecular mechanisms of Salvia Miltiorrhiza in treating rheumatoid arthritis based on network pharmacology.Methods The traditional Chinese medicine systems pharmacology database and analysis platform(TCMSP)was used to obtain the active ingredients and targets of Salvia Miltiorrhiza,which were mapped with the rheumatoid arthritis disease gene targets obtained from GeneCards and OMIM databases,to construct a drug-active ingredient-disease target network.Gene ontology(GO)functional enrichment,and Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis were performed in the DAVID database.Results A total of 56 active ingredients and 100 target genes were screened from Salvia Miltiorrhiza.A total of 1640 target genes for rheumatoid arthritis disease were retrieved.A total of 44 common targets were obtained from the intersection of Salvia Miltiorrhiza and rheumatoid arthritis disease target genes,of which 13 were key targets.A total of 96 GO biological processes and 25 KEGG signaling pathways were obtained,involving immune regulation,inflammatory response,and apoptosis.Conclusion Salvia Miltiorrhiza can be used to treat rheumatoid arthritis through a multi-component and multi-target network.
作者
李自霖
王桂花
张翠香
陈贵元
李雪英
LI Zilin;WANG Guihua;ZHANG Cuixiang;CHEN Guiyuan;LI Xueying(Department of Biochemistry and Molecular Biology,School of Basic Medical Sciences,Dali University,Yunnan,Dali 671000,China;Department of Respiratory and Critical Care Medicine,the First Affiliated Hospital of Dali University,Yunnan,Dali 671000,China)
出处
《中国医药科学》
2024年第9期91-95,共5页
China Medicine And Pharmacy
基金
国家自然科学基金项目(31860252)
云南省自然科学基金高校联合面上项目(2017FH001-084)。
关键词
网络药理学
丹参
类风湿关节炎
分子机制
Network pharmacology
Salvia Miltiorrhiza
Rheumatoid arthritis
Molecular mechanism
