摘要
目的建立橘红胎饮片标准汤剂的质量评价体系,并对其进行质量综合评价。方法制备橘红胎饮片标准汤剂,以出膏率、柚皮苷和野漆树苷转移率及高效液相(HPLC)指纹图谱相似度为评价指标,建立其质量评价体系;并采用灰色关联法对指纹图谱中标定成分进行关联分析,评价16批橘红胎饮片标准汤剂的质量。结果 16批橘红胎饮片中柚皮苷含量为3.34%~29.27%,野漆树苷含量为0.26%~2.82%。橘红胎标准汤剂的出膏率范围为43.36%~51.52%;柚皮苷和野漆树苷的转移率范围为33.62%~144.86%和1.30%~96.00%;指纹图谱确定共有峰19个,其中4个峰指认为柚皮苷(8号)、新橙皮苷(11号)、野漆树苷(12号)、柚皮素(15号);16批标准汤剂指纹图谱相似度均在0.98以上。指纹图谱中4个标定成分的灰色关联度范围为0.096~0.606,加权关联度范围为0.091~0.984。结论该研究建立的标准汤剂质量评价体系中各指标范围均合理可靠,运用指纹图谱中标定成分的灰色关联分析能够实现16批橘红胎饮片标准汤剂的质量综合评价。
Objective To establish the evaluation system and accomplish the comprehensive evaluation of the quality of Juhongtai standard decoction.MethodsThe standard decoction of Juhongtai was prepared. The extract rates and transfer rates of naringin and rhoifolin were measured and calculated. The high performance liquid chromatography(HPLC) fingerprint was also established and the similarities of the fingerprints of 16 batches of decoctions were calculated. These parameters were selected as evaluation indicators of the quality evaluation system. Moreover,the identified components in the fingerprint were correlated using grey correlation statistics to evaluate the quality of the standard decoctions of 16 batches of Juhongtai.ResultsThe contents of naringin and rhoifolin in 16 samples were3.34%-29.27% and 0.26%-2.82%,respectively. The transfer rates were 43.36%-51.52%,while extract rates of naringin and rhoifolin were 33.62%-144.86% and 1.30%-96.00%, respectively. According to the Similarity Evaluation System for Chromatographic Fingerprint of traditional Chinese medicine(TCM),there were 19 common peaks in all chromatograms in which 4 peaks were identified as naringin(8),neohesperidin(11),rhoifolin(12)and naringenin(15), respectively. The similarities of 16 samples were all greater than 0.98. In addition, the grey correlation grade of the 4 identified components in 16 samples was 0.096-0.606,and the weighted grey correlation grade was 0.091-0.984.ConclusionThis study established a reasonable and reliable quality evaluation system for standard decoction of Juhongtai. Besides,the quality of the standard decoction of Juhongtai can be comprehensively evaluated by the grey correlation analysis of the identified components of the fingerprint.
作者
张晓营
文廷芳
张雅洁
方家祺
麦宝愉
肖凤霞
ZHANG Xiaoying;WEN Tingfang;ZHANG Yajie;FANG Jiaqi;MAI Baoyu;XIAO Fengxia(School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine,Guangzhou 510006 Guangdong,China;Guangdong Lewwin Pharmaceutical Research Institute Co.,Ltd.,Guangzhou 510006 Guangdong,China)
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2021年第3期406-413,共8页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
国家中医药管理局中药标准化项目(ZYBZH-Y-GD-13)
广州中医药大学薪火计划项目(A1-AFD018181Z4259)
以农产品为单元的广东省现代农业产业技术体系创新团队建设项目(南药产业,2019KJ148)
广东省岭南中药材保护资金人才培养项目(粤中医办函[2019]32号、144号)
渔港建设和渔业产业发展专项(海洋渔业科技推广方向-科技攻关与研发项目,粤财农[2017]17号)。
关键词
橘红胎
标准汤剂
指纹图谱
灰色关联度
质量评价
Juhongtai
standard decoction
fingerprint
grey correlation analysis
quality evaluation
