摘要
目的观察山药多糖治疗肥胖糖尿病肾病大鼠的效果,并探讨其对肾功能、肠道微生态的影响。方法以高脂饮食、肾切除+腹腔注射STZ建立肥胖糖尿病肾病大鼠模型,分为5组,另取8只正常SD大鼠记为正常组。阳性药组予以10 mg/kg洛丁新灌胃,低剂量组、中剂量组和高剂量组分别予以50、100、200 mg/kg山药多糖灌胃,模型组与正常组均予以等量生理盐水灌胃,每天1次,共30 d。对比治疗前后体质量、尿蛋白、肾功能、肠道菌群变化。结果治疗后阳性药组和3剂量组体质量、尿蛋白、血清肌酐(Scr)、血清尿素氮(BUN)水平均下降,且均低于模型组,模型组则均高于正常组,差异均有统计学意义(P<0.05);治疗后模型组厚壁菌门、毛螺菌属、韦荣球菌属、芽孢杆菌属、类芽孢杆菌属、酸梭菌属相对丰度下降,阳性药组和山药多糖3剂量组则升高,且治疗后模型组厚壁菌门、毛螺菌属、韦荣球菌属、芽孢杆菌属、类芽孢杆菌属、酸梭菌属相对丰度均低于正常组;拟杆菌门、变形菌门、拟杆菌属、埃希菌属、志贺菌属、沙门菌属相对丰度及治疗后组间变化趋势相反,上述差异均有统计学意义(P<0.05),且山药多糖的作用呈剂量依赖性。LEfSe结果显示Ruminococcaceae、unclassified-Lanchnospiraceae、unclassified-Firmicutes-family、unclassified-Firmicutes-sensu-stricto等是biomarker。结论山药多糖可减轻肥胖糖尿病肾病大鼠的体重,改善肾功能,还可调节肠道微生态,其作用呈剂量依赖性。
Objective To observe the effect of yam polysaccharides in the treatment of obese diabetic nephropathy rats,and explore its effect on renal function and intestinal microecology.Methods The rat models of obese diabetic nephropathy were established using high fat diet,nephrectomy and intraperitoneal injection of STZ,and divided into 5 groups.Another 8 normal SD rats were recorded as normal group.The positive group was given 10 mg/kg of lodine by gavage,while the low dose group,medium dose group and high dose group were given 50,100 and 200 mg/kg of yam polysaccharides by gavage respectively,qd for 30 days.The model group and the normal group were given the same amount of normal saline by gavage.The changes of body mass,urine protein,renal function and intestinal microecology before and after intervention were compared among groups.Results After treatment,the body mass,urine protein,serum creatinine(SCR)and serum urea nitrogen(BUN)in the positive drug group and the three dose groups decreased,and all of them were lower than those in the model group,while those in the model group were higher than those in the normal group,and the differences were statistically significant(all P<0.05).The relative abundances of Firmicutes,Lachnospira,Veillonella,Bacilus,Paenibacillus and Acidobacteria in the model group after treatment decreased,while those in the positive group and the 3 dose groups increased.The relative abundances of Firmicutes,Lachnospira,Veillonella,Bacilus,Paenibacillus and Acidobacteria in the model group after treatment were lower than those in the normal group.The relative abundances of Bacteroidetes,Proteobacteria,Bacteroides,Escherichia,Shigella and Salmonella after treatment changed oppositely among groups.The differences were statistically significant(all P<0.05).The effect of yam polysaccharides was dose-dependent.LEfSe analysis showed that the biomarkers were Ruminococcaceae,unclassified-Lanchnospiraceae,unclassified-Firmicutes-family,unclassified-Firmicutes-sensu-stricto and so on.Conclusion Yam polysaccharides have a good effect on obese diabetic nephropathy rats,which can reduce body weight,improve renal function,and regulate intestinal microecology in a dose-dependent way.
作者
张文杰
赖星海
陈佳薇
ZHANG Wen-jie;LAI Xing-hai;CHEN Jia-wei(Nephrology,People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350004,China;不详)
出处
《中国微生态学杂志》
CAS
CSCD
2021年第1期37-42,共6页
Chinese Journal of Microecology
关键词
山药多糖
肥胖
糖尿病肾病
肾功能
肠道菌群
Yam polysaccharide
Obese
Diabetic nephropathy
Renal function
Intestinal microflora
