摘要
种子活力高低直接影响大豆出苗质量,进而影响大豆的高产与稳产。为应对近年来东北地区低温胁迫对大豆种子活力的影响日趋严重的问题,筛选低温胁迫下高活力的种质资源,本研究鉴定335份大豆资源芽期的耐低温性,分别设置6℃和20℃处理,测定发芽势、发芽率、发芽指数及各指标的差异性和相关性,利用隶属函数法对这些资源进行综合评价。结果表明:6℃低温处理降低大豆种子的发芽势、发芽率和发芽指数,且各指标在品种间差异显著(P<0.01);相关性分析结果表明发芽势、发芽率和发芽指数彼此间在0.01水平上呈显著正相关,且相关系数均大于0.8;隶属函数综合评价结果表明,335份大豆资源耐低温性存在不同程度差异;聚类分析可将品种分为3类:合农97、吉林小粒豆CK和黑农69等17个品种耐低温性较强;合农76、SB8842和垦鉴豆43等161个品种耐低温性次之;公05163-3、华庆豆103和龙黑大豆1号等157个品种耐低温性差。研究结果将为耐低温大豆品种的选育提供材料基础和理论依据。
Seed vigor directly affects the quality of soybean seedling,and then affects the high and stable yield of soybean.In order to deal with the problem of low temperature stress on soybean seed vigor in northeast China in recent years,and screen the germplasm resources with high vigor under low temperature stress,in this study,the low temperature resistance of the budding stage of 335 soybean resources were identified and treated at 6℃and 20℃respectively.The germination energy,germination rate and germination index were determined,and the difference and correlation of indexes were analyzed.Then,the membership function method was used to comprehensively evaluate these resources.The results showed that:6℃low temperature treatment reduced the germination energy,germination rate and germination index of soybean seeds,each index was significantly different among different varieties.The results of correlation analysis showed that the germination energy,germination rate and germination index were significantly positively correlated at the level of 0.01,and the correlation number was greater than 0.8.The comprehensive evaluation of membership function and cluster analysis results showed that 335 soybean varieties had significant differences.The cluster analysis of 335 soybean resources could be divided into three categories:17 varieties,such as Henong 97,Jilinxiaolidou CK and Heinong 69,had strong low temperature tolerance,161 varieties,such as Henong 76,sb8842 and Kenjiandou 43 were the second,157 varieties such as Gong 05163-3,Huaqingdou 103 and Longheidadou 1 had poor low temperature tolerance.These results would provide material and theoretical basis for breeding of low temperature resistant soybean varieties.
作者
郑莉萍
张云峰
蒋洪蔚
王明亮
邱红梅
孙星邈
朴锦
王曙明
ZHENG Li-ping;ZHANG Yun-feng;JIANG Hong-wei;WANG Ming-liang;QIU Hong-mei;SUN Xing-miao;PIAO Jin;WANG Shu-ming(Agronomy College,Yanbian University,Yanji 133002,China;Soybean Research Institute,Jilin Academy of Agricultural Sciences/National Engineering Research Center for Soybean,Changchun 130033,China)
出处
《大豆科学》
CAS
CSCD
北大核心
2020年第6期833-847,共15页
Soybean Science
基金
吉林省农业科技创新工程人才基金—研究生基金(c02100413)
吉林省农业科技创新工程定向委托项目(CXGC2020DX003)
2019年度省科研院所引进高层次科技创新人才资助计划
现代农业产业技术体系建设专项(CARS-04-PS11)。
关键词
大豆
芽期
低温胁迫
萌发
隶属函数法
聚类分析
Soybean
Bud stage
Low temperature stress
Germination
Membership function method
Cluster analysis
