摘要
为筛选适宜黑龙江省推广种植的耐冷性大豆种质资源,以152份适于黑龙江省种植的大豆种质为材料进行15℃低温萌发处理,调查低温胁迫下大豆萌发期的相关指标。并对15℃胁迫下表现出耐冷特性的6份大豆种质进行6℃低温处理,调查其相对发芽率。结果表明,在15℃胁迫下不同种质萌发期耐冷性存在丰富的变异;相关性分析表明,发芽率、发芽势和发芽指数呈极显著正相关。通过隶属函数和聚类分析对不同种质萌发期耐冷性进行综合性评价,将152份参试种质分为三类,第一类为耐冷种质19份;第二类为中等耐冷种质51份;第三类为低温敏感种质82份。在6℃下的6份参试种质按照相对发芽率分级标准可分为3个等级,Ⅰ级耐冷种质2份;Ⅱ级中等耐冷种质3份;Ⅲ级敏感种质1份。综合15和6℃下的分类结果筛选出2份耐冷种质,为蒙豆14×711和蒙豆16×冀豆17。
In order to screen out cold-tolerant soybean germplasm resources, 152 soybean germplasms suitable for planting in Heilongjiang Province were germinated at 15 ℃,and the related indexes of soybean germination period under low temperature stress were investigated in this study.Six soybean germplasms with cold tolerance at 15 ℃ were treated at 6 ℃ to investigate their relative germination rate.The results showed that there were abundant variations in cold tolerance of different germplasms during germination at 15 ℃.Correlation analysis showed that germination rate, germination potential and germination index were significantly positively correlated.The cold tolerance of different germplasms at germination stage was comprehensively evaluated by membership function and cluster analysis.152 germplasms were divided into three categories, the first category was 19 cold tolerance germplasms, the second category was 51 medium cold tolerance germplasm and the third category was 82 low temperature sensitive germplasm.The 6 tested germplasms at 6 ℃ could be divided into 3 grades according to the relative germination rate classification standard, and 2 germplasms with grade Ⅰ cold tolerance.Grade Ⅱ medium cold tolerance germplasm 3,and one grade Ⅲ sensitive germplasm.Based on the classification results at 15 and 6 ℃,two cold-tolerant germplasms were selected as Mengdou 14×711 and Mengdou 16×Jidou 17.
作者
谢路
李健平
韩德志
谷勇哲
邳植
XIE Lu;LI Jian-ping;HAN De-zhi;GU Yong-zhe;PI Zhi(College of Resources and Environment,Heilongjiang University,Harbin 150080,China;Heihe Branch,Heilongjiang Academy of Agricultural Sciences,Heihe 164300,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China)
出处
《黑龙江农业科学》
2022年第7期1-7,共7页
Heilongjiang Agricultural Sciences
基金
中国和乌拉圭联合实验室合作项目(2018YFE0116900)
大豆种质资源保护与利用项目(19211205)。
关键词
大豆
萌发期
耐冷性
种质资源鉴定
soybean
germination
tolerance to cold stress
identification of germplasm resources
