摘要
木薯蚕丝作为一种极具开发利用价值的野蚕丝,其结晶结构、热学性质和力学性能显著不同于桑蚕丝,对木薯蚕丝二级结构的研究有助于进一步认识其构效关系,拓展其应用领域。13C CP/MAS NMR技术是研究蚕丝丝素蛋白结构的有效方法之一。文章通过13C CP/MAS NMR测试,分析了木薯蚕丝丝素蛋白的二级结构特征,并将其与桑蚕丝和柞蚕丝进行了对比。结果表明:桑蚕丝中Gly的含量较柞蚕丝和木薯蚕丝高,木薯蚕丝与柞蚕丝相近;Ala的含量及其β-sheet结构较柞蚕丝和木薯蚕丝低,木薯蚕丝最高,三者的α-螺旋结构相差不大;桑蚕丝的不定形区含量最高,木薯蚕丝最低,与柞蚕丝接近。
Cassava silk as a kind of wild silk with great development and utilization value,is significantly different from mulberry silk in terms of the crystal structure,thermal properties and mechanical properties.The study on the secondary structure of cassava silk can help to further understand its structure-activity relationship and expand its application field.13C CP/MAS NMR is an effective method to study the structure of silk fibroin.By 13C CP/MAS NMR test,the secondary structure characteristics of cassava silk were analyzed,and then compared with those of mulberry silk and tussah silk.The results showed that the content of Gly in mulberry silk was higher than that in tussah silk and cassava silk,and the content of Gly in tussah silk and cassava silk was close.The content of Ala andβ-sheet structure were lower than those of tussah silk and cassava silk,which were the highest in cassava silk.There was no significant difference inα-helix structure among the three.The content of amorphous region in mulberry silk was the highest,and that of cassava silk was the lowest,which was close to tussah silk.
作者
陈静
黄继伟
彭泽冶
宁晚娥
CHEN Jing;HUANG Jiwei;PENG Zeye;NING Wan’e(College of Biological and Chemical Engineering,Guangxi University of Science and Technology,Liuzhou 545006,China;College of Textile and Clothing Engineering,Soochow University,Suzhou 215123,China)
出处
《丝绸》
CAS
CSCD
北大核心
2021年第1期1-7,共7页
Journal of Silk
基金
国家自然科学基金项目(51763001,51963002)
广西自然科学基金项目(2018GXNSFAA281280)
柳州市科技计划项目(2019DH10602)。
