摘要
为研究芦丁联合花青素对肾细胞氧化损伤的修复效果,该文以HEK293细胞株建立尿酸诱导的肾细胞氧化损伤模型。用CCK-8法测定受损细胞的活性,并利用Hochest33342(10 μg/mL)与PI(10 μg/mL)对细胞进行染色,观察其凋亡情况。最后借助氧化应激试剂盒检测细胞内活性氧(reactive oxygen species,ROS)含量的变化。实验结果分析表明,尿酸可诱导HEK293细胞产生氧化损伤,且以含1.4 g/L尿酸的培养基孵育细胞24 h可建立最佳的HEK293细胞损伤模型。芦丁与花青素对损伤细胞均有一定修复效果,且在两者联用时(芦丁40 μg/mL、花青素40 μg/mL)可得到最佳修复效果(60%修复率)。该研究证明了芦丁和花青素联合作用能够对氧化损伤的肾细胞起到较好的修复作用。
In order to study the repair effect of rutin combined with anthocyanin on oxidative damage of renal cells,a uric acid-induced oxidative damage model of renal cells was established using HEK293 cell line.The activity of the damaged cells was determined by CCK-8 method.Hochest33342 (10 μg/mL) and PI (10 μg/mL) were used to stain the cells and observe their apoptosis.Finally,the change of intracellular ROS (reactive oxygen species) content was detected with the aid of the oxidative stress kit.The results showed that uric acid could induce oxidative damage of HEK293,and the optimal HEK293 cell damage model could be established by incubating the cells in a medium containing 1.4 g/L uric acid for 24 h.Both rutin and anthocyanin had certain repair effect on damaged cells,and the optimal repair effect (60% repair rate) was obtained when the two were used together (40 μg/mL rutin and 40 μg/mL anthocyanin).This study proves that the combined action of rutin and anthocyanin can play a better role in the repair of oxidative damaged renal cells.
作者
刘剑
周瑜桥
徐梦姣
杨昱祺
曲道峰
LIU Jian;ZHOU Yuqiao;XU Mengjiao;YANG Yuqi;QU Daofeng(Zhejiang Gongshang University,Hangzhou 310018,China)
出处
《中国细胞生物学学报》
CAS
CSCD
2020年第10期1732-1737,共6页
Chinese Journal of Cell Biology
基金
全面实施高等教育强省战略背景下高校伙食物资联合采购工作的研究项目(批准号:5030KZ0420005)资助的课题。
关键词
肾细胞
氧化损伤
修复
芦丁
花青素
renal cells
oxidative damage
repair
rutin
anthocyanin
