摘要
目的研究血液中的卤代羟基烷类吸入用全身麻醉剂对血液中乙醇含量检测的影响。方法利用顶空气相色谱双柱确认检测方法对卤代羟基烷类进行分析,观察其在KB-BAC1/KB-BAC2和J&W DBALC1/DB-ALC2气相色谱柱条件下对顶空气相色谱法检测血液中乙醇含量的影响。结果J&W DBALC1/DB-ALC2气相色谱柱中,七氟烷、恩氟烷分别与乙醇和叔丁醇的保留时间相近,对血液中乙醇含量的检测存在干扰;仅J&W DB-ALC1气相色谱柱可分离七氟烷和乙醇成分,以排除其对血液中乙醇含量检测的影响。在KB-BAC1/KB-BAC2气相色谱柱中,七氟烷、异氟烷与乙醇的保留时间相近,尤其是七氟烷与乙醇的保留时间基本一致,对血液中乙醇含量检测存在明显干扰。结论卤代羟基烷类对顶空气相色谱法检测血液中乙醇含量存在干扰,选择适当的色谱柱进行双柱确认检测可有效予以甄别。
Objective To study the influence of halogenated hydroxyl-alkanes inhalation anesthetic on the determination of ethanol content in blood.Methods Halogenated hydroxyl-alkanes were analyzed by headspace gas chromatography with double column confirmatory detection method.The influence of halogenated hydroxyl-alkanes on determination of ethanol content in blood sample by headspace gas chromatography was explored under the different detection conditions of KB-BAC1/KB-BAC2 and J&W DB-ALC1/DB-ALC2 gas chromatographic column.Results The retention time of sevoflurane and enflurane was similar to that of ethanol and tert butanol respectively when using the J&W DB-ALC1/DB-ALC2 gas chromatographic column,and interfered with the detection of ethanol content in blood;only J&W DB-ALC1 gas chromatographic column can separate the sevoflurane and ethanol components,so as to eliminate their influence on the detection of ethanol content in blood.When using KB-BAC1/KB-BAC2 gas chromatographic column,the retention time of sevoflurane,isoflurane and ethanol is similar,especially that of sevoflurane and ethanol,and sevoflurane obviously interferes with the determination of ethanol content in blood.Conclusion Halogenated hydroxy-alkanes interfere with determination of ethanol content in blood by headspace gas chromatography.The interference can be discriminated effectively by choosing the suitable chromatographic column and double column confirmatory detection.
作者
谢冰
闫文静
孟宪跃
苗鑫刚
于峰
董玫
施妍
向平
马春玲
文迪
XIE Bing;YAN Wen-jing;MENG Xian-yue;MIAO Xin-gang;YU Feng;DONG Mei;SHI Yan;XIANG Ping;MA Chun-ling;WEN Di(Collaborative Innovation Center of Forensic Medical Molecular Identification,Hebei Key Laboratory of Fo-rensic Medicine,College of Forensic Medicine,Hebei Medical University,Shijiazhuang 050017,China;Huang-hua Forensic Identification Center,Huanghua 061100,Hebei Province,China;Shijiazhuang Public Trans-portation Administration Bureau,Shijiazhuang 050091,China;Shanghai Key Laboratory of Forensic Medi-cine,Key Laboratory of Forensic Science,Ministry of Justice,Shanghai Forensic Service Platform,Academy of Forensic Science,Shanghai 200063,China)
出处
《法医学杂志》
CAS
CSCD
2020年第5期682-687,共6页
Journal of Forensic Medicine
基金
国家重点研发计划资助项目(2018YFC0807204,2016YFC0800704)。
关键词
法医毒理学
卤代羟基烷
麻醉药
吸入
乙醇
顶空气相色谱法
血液
forensic toxicology
halogenated hydroxy-alkanes
anesthetics,inhalation
ethanol
headspace gas chromatography
blood
