摘要
建立液相色谱串联质谱法测定核桃、杏仁、大豆和花生中的蛋白成分,并定量检测核桃露和杏仁露中常见外源成分大豆和花生的含量。样品经蛋白质提取,胰蛋白酶酶解后,采用高效液相色谱-四极杆/静电场轨道阱高分辨质谱(high performance liquid chromatography coupled with quadrupole-exactive mass spectrometry,HPLC-Q-Exactive-MS)检测,数据经Proteome Discoverer软件与Uniprot蛋白数据库对比分析,筛选出各物种的特征肽段,然后利用高效液相色谱-三重四极杆质谱(high performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry,HPLC-QQQ-MS)对核桃、杏仁、大豆和花生的特征多肽进行验证和多反应监测(multiple reaction monitoring,MRM)定量测定。经验证,鉴别方法有良好的专属性和高灵敏度,核桃露和杏仁露中核桃、杏仁、大豆和花生的最低检出限分别为16.3、7.7、1.3和6.8 mg/kg。该方法准确、灵敏,可有效分析植物蛋白饮料中各类蛋白质的来源,对食品植物源性成分的鉴定具有重要意义。
A liquid chromatography-mass spectrometry method was established for the identification of proteins from walnuts,almonds,soybeans and peanuts,and quantitative detection of common exogenous ingredients such as soybean and peanut in walnut drink and almond drink.The samples were determined by ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry(HPLC-Q-Exactive-MS)after protein extraction and tryptic digestion.The peptide markers of each species were obtained by data analysis with Proteome Discoverer software and Uniprot protein database,and then the verification and multiple reaction monitoring(MRM)of the marker peptides of walnut,almond,soybean and peanut were performed quantitatively using high performance liquid chromatography-triple quadrupole mass spectrometry(HPLC-QQQ-MS).The results were verified and the identification method had good specificity and high sensitivity.The limit of detection of walnut,almond,soybean and peanut in walnut and almond drink were 16.3 mg/kg,7.7 mg/kg,1.3 mg/kg and 6.8 mg/kg respectively.This method is accurate and sensitive,and can effectively analyze the origin of proteins in plant protein beverages.And this method is important for identification of plant-derived ingredients in food.
作者
张淑霞
田亚
邢荣花
刘胜男
王向军
张守杰
ZHANG Shuxia;TIAN Ya;XING Ronghua;LIU Shengnan;WANG Xiangjun;ZHANG Shoujie(Zhengzhou Customs Technical Center,Zhengzhou 450002,China;Anyang Customs,Anyang 455000,China;Sanmenxia Customs Technical Center,Sanmenxia 472000,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2020年第21期215-222,共8页
Food and Fermentation Industries
基金
海关总署科技计划项目(2017IK283,2017IK284)。
关键词
液相色谱串联质谱
特征肽段
植物蛋白饮料
掺假测定
外源成分
liquid chromatography-tandem mass spectrometry
marker peptides
vegetable protein beverages
adulteration detection
exogenous ingredients
