摘要
【目的】研究赤霉素(gibberellin)对植物株高的影响,探讨了GA20ox、GA3ox和GA2ox在赤霉素合成过程中的反馈调节机制。【方法】以薄壳山核桃Carya illinoensis幼苗为材料,进行100 mg·L^−1赤霉素叶面喷施处理。对外施赤霉素后薄壳山核桃的株高、节间长度和主根长以及薄壳山核桃GA20ox、GA3ox和GA2ox的时空变化进行了研究。【结果】外施赤霉素28 d后,薄壳山核桃节间长度和主根长的伸长量与对照相比都存在显著差异(P<0.05),节间数量没有发生变化;植株平均生长量达2.9 cm,为对照的1.93倍,差异极显著(P<0.01)。实时荧光定量PCR结果表明:GA20ox、GA3ox和GA2ox在薄壳山核桃生长期存在时空表达差异。外施赤霉素能够使CiGA20ox的表达量持续下降,28 d后的表达量仅为初始值的38.6%;而CiGA3ox表达量则是在7 d时跌落低谷,下降至初始值的55.4%,28 d后回升,表达量为初始值的350%;CiGA2ox表达量总体呈波浪形变化,在7 d后达到顶峰,之后有所回落,至21 d时恢复到了初始水平,28 d后上升为初始值的220%。CiGA3ox在薄壳山核桃植株茎杆内发生了累积,相较于CiGA20ox和CiGA2ox在转录水平上发生了较大的变化。【结论】外施赤霉素促进了薄壳山核桃茎杆伸长,同时引起了薄壳山核桃体内赤霉素代谢关键基因表达模式的时空变化。
[Objective]This study attempts to explore the effect of gibberellin(GA3)on plant height and discuss the feedback regulation mechanism of GA20ox,GA3ox and GA2ox in the process of GA3 synthesis.[Method]The leaves of Carya illinoensis seedlings were sprayed with 100 mg·L^−1 GA3.The plant height,internode length and main root length of C.illinoensis and the spatiotemporal variation of CiGA20ox,CiGA3ox and CiGA2ox were studied.[Result]After 28 days of GA3 application,the internode length and the elongation of the main root length of C.illinoensis seedlings were significantly different from those of the control(P<0.05),but the number of internodes did not change.The average plant growth was 2.9 cm,which was 1.93 times that of the control,and the difference was extremely significant(P<0.01).The results of real-time PCR showed that there were spatial-temporal differences in the expression of GA20ox,GA3ox and GA2ox during the growth period of C.illinoensis.Exogenous GA3 could continuously reduce the expression of CiGA20ox.After 28 days,the expression level of CiGA20ox was only 38.6%of the initial value.The expression level of CiGA3ox dropped to a low point of 55.4%of the initial value on day 7 and rose to 350%of the initial value on day 28.The expression level of CiGA2ox in general showed a wavy change,reaching the peak after 7 days,then falling back to the initial level after 21 days,and rising to 220%of the initial value after 28 days.However,CiGA3ox accumulated in the stem of C.illinoensis seedlings,which changed in transcription level compared with CiGA20ox and CiGA2ox.[Conclusion]The exogenous GA3 could promote the stem elongation of C.illinoensis and cause the temporal and spatial variation of the key gene expression patterns of GA3 metabolism in C.illinoensis.
作者
严泽埔
张佳琦
梁璧
魏广利
张启香
王正加
YAN Zepu;ZHANG Jiaqi;LIANG Bi;WEI Guangli;ZHANG Qixiang;WANG Zhengjia(State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China)
出处
《浙江农林大学学报》
CAS
CSCD
北大核心
2020年第5期922-929,共8页
Journal of Zhejiang A&F University
基金
国家自然科学基金资助项目(31670682)
浙江省科技厅重大研发专项(2018C02004)
浙江省农业新品种选育重大科技专项(2016C02052-13)
中央财政林业科技推广项目(〔2018〕TS 08号)。
关键词
林木育种学
薄壳山核桃
赤霉素
赤霉素氧化酶基因
时空变化
forest tree breeding
Carya illinoensis
gibberellins(GA3)
gibberellin oxidase gene
temporal and spatial variation
