摘要
【目的】揭示不同栽培环境对铁皮石斛Dendrobium catenatum内生细菌群落结构的影响,解析与石斛多糖含量存在潜在关联的功能菌种。【方法】针对温室人工基质培养和仿野生立木附生培养2种栽培体系下的铁皮石斛C9品系根、茎、叶等各部组织内生细菌,采用Illumina测序平台的Miseq进行高通量测序分析其菌落结构和多样性,同时利用逐步回归法分析内生细菌分布与组织多糖质量分数的关联性。【结果】铁皮石斛在2种栽培环境不同组织中的菌群结构差异显著(P<0.05),附生立木仿野生环境比温室环境下物种丰富度更高。同时,根部所含分类操作单元(OTUs)数量最多(112~630个),叶部则最少(28~73个)。其中,厚壁菌门Firmicutes(16.7%~34.9%)、拟杆菌门Bacteroidetes(8.7%~35.6%)和变形菌门Proteobacteria(15.9%~42.0%)的OTUs占优势。从菌种生理功能上看,固氮相关的菌种如弗兰克菌科Frankiaceae在树培环境下相对丰度更高,推测可能受树培寡氮环境的影响使固氮菌富集。逐步回归分析结果显示:不动杆菌Acinetobacter在根、茎、叶不同组织中的相对丰度与多糖质量分数变化关联度最大,其次为假单胞菌Pseudomonas。【结论】铁皮石斛内生细菌资源相当丰富,而栽培方式影响菌群分布,且与石斛多糖质量分数具有关联性。
[Objective]Aimed to discover the effect of different cultivation measures on endophytic bacterial communities and polysaccharides accumulation in Dendrobium catenatum,this research is conducted to compare D.catenatum in two environments of soil cultivation in green house and truck-attaching cultivation in open field.[Method]With the Miseq high-throughput sequencing on Illumina platform conducted,efforts are made to discover the community structures,diversity,and potential functions of bacterial symbionts in different tissues such as roots,stems and leaves of D.catenatum.Besides,the correlation of polysaccharide content with bacterial community was also analyzed employing the stepwise method.[Result](1)There is significant difference(P<0.05)in bacterial communities in different tissues with different cultivation environments;(2)The bacterial richness was higher in truck-attaching cultivation than that in greenhouse,and the operational taxonomic units(OTUs)in root tissues are the largest in number(112-630 OTUs)while those in leaf tissues(28-73 OTUs)are the smallest in number.Comparatively,the OTUs in phyla of Firmicutes(16.7%-34.9%),Bacteriodetes(8.7%-35.6%)and Proteobacteria(15.9%-42.0%)dominate in tissues;(3)Physiologically,OTUs of nitrogen-fixation related bacteria such as Frankiaceae are larger in number in truck-attaching cultivation environment,reflective of the influence of nitrogen-lacking environment.The stepwise procedure results have shown that the relative abundance of Acinetobacter has the highest correlation with the polysaccharide contents in different tissues of root,stem and leaf,followed by Pseudomonas.[Conclusion]D.catenatum are rich in endophytic bacteria,cultivation measures exert effect on the bacterial community and some bacteria are correlated with the polysaccharides contents.These results shall shed light on further exploration of endophytic bacterial resources in D.catenatum.
作者
余望寅
姚冠
扎西拉姆
普琼
刘京晶
周湘
YU Wangyin;YAO Guan;Tashi Lhamo;Phur Qiong;LIU Jingjing;ZHOU Xiang(State Key Laboratory of Subtropical Silviculture,School of Forestry&Biotechnology,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China)
出处
《浙江农林大学学报》
CAS
CSCD
北大核心
2020年第2期284-290,共7页
Journal of Zhejiang A&F University
基金
国家自然科学基金资助项目(81603228)
浙江农林大学学生科研训练项目(KX20180046)。
关键词
林木育种学
植物内生细菌
多样性
固氮
菌群分析
石斛多糖
forest tree breeding
endophytic bacteria
diversity
nitrogen fixation
bacterial community analysis
Dendrobium polysaccharides
