摘要
目的观察滋肾活血方对血管性痴呆(vascular dementia, VD)大鼠海马组织自噬标记蛋白LC3Ⅱ、Beclin1以及自噬调控PINK1/Parkin信号通路的影响。方法改良2-VO法建立VD大鼠模型,采用滋肾活血方进行干预,免疫组化法检测VD大鼠海马组织LC3Ⅱ、Beclin1、PINK1以及Parkin蛋白的表达。结果滋肾活血方能显著提高VD大鼠海马组织自噬标记蛋白LC3Ⅱ、Beclin1以及自噬调控PINK1/Parkin信号分子的表达(P<0.01)。结论滋肾活血方可能通过激活PINK1/Parkin信号通路,促进细胞自噬,保护VD大鼠海马神经元,改善其学习记忆能力。
Objective To observe the effects of Zishen Huoxue Decoction on the autophagy related protein LC3Ⅱ and Beclin1, and autophagy regulation PINK1/Parkin signaling molecules in hippocampal of rats with vascular dementia(VD). Methods The modified 2-VO method was used to establish the VD rat model. The expressions of LC3Ⅱ, Beclin1, PINK1 and Parkin proteins in the hippocampal of VD rats were detected by immunohistochemistry after the intervention with Zishen Huoxue Decoction.Results The expression of LC3Ⅱ, Beclin1 and autophagy regulation PINK1/Parkin signaling molecules in the hippocampal of VD rats were significantly improved after the intervention with Zishen Huoxue Decoction(P<0.01). Conclusion Zishen Huoxue Decoction may improve autophagy, protect hippocampal neurons of VD rats, and improve learning and memory ability by activating PINK1/Parkin signaling pathway.
作者
谢乐
伍大华
曹思佳
任晨斌
刘涵
XIE Le;WU Dahua;CAO Sijia;REN Chenbin;LIU Han(Neurology Department,The Affiliated Hospital of Hunan Academy of Chinese Medicine,Changsha,Hunan 410006,China;School of Humanities and Management,Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;Internal Medicine Department,Ningbo Beilun District Hospital of Traditional Chinese Medicine,Ningbo,Zhejiang 315800,China;Graduate School,Hunan University of Chinese Medicine,Changsha,Hunan 410208,China)
出处
《湖南中医药大学学报》
CAS
2020年第9期1082-1085,共4页
Journal of Hunan University of Chinese Medicine
基金
国家自然科学基金面上项目(81874462)
湖南省自然科学基金科卫联合项目(2018JJ6022)。
