摘要
目的研究杜仲多糖(EUP)对2型糖尿病db/db小鼠糖脂代谢的影响及相关作用机制。方法采用db/db 2型糖尿病小鼠模型,随机分成模型组、EUP低、中、高剂量组以及阳性对照二甲双胍组,另设同周龄-+/db小鼠为正常对照组,给药9周后称量小鼠体质量,检测小鼠空腹血糖(FBG)、血脂、白细胞介素-8(IL)-8、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GSH-Px)和丙二醛(MDA)的含量变化;HE染色和油红O染色评估小鼠肝脏病理学变化及脂肪变性;Western-blot检测小鼠肝脏组织核因子(NF)-κB p65和血红素加氧酶(HO-1)蛋白表达。结果EUP能够减轻自发性2型糖尿病db/db小鼠的体质量,降低FBG、TG、TC、LDL-C、IL-8、IL-1β、IL-6、MDA和NF-κB p65蛋白水平,提高HDL-C、GSH-Px、SOD和HO-1蛋白水平;EUP能够减轻db/db小鼠肝脏的病理改变和脂肪变性。结论EUP对2型糖尿病db/db小鼠表现的肥胖、高血糖、高血脂和肝损伤均有较好的改善作用,其机制跟调控炎症反应及氧化应激水平有关。
OBJECTIVE To investigate the effect and mechanism of Eucommia ulmoides polysaccharide(EUP)on db/db diabetic mice′s glycolipid metabolism.METHODS The db/db type 2 diabetes mice model were randomly divided into model control group,EUP low,middle and high dose group and positive control metformin group,-+/db mice of the same age were set as normal control group.The mice were weighed,and the changes of fasting blood glucose(FBG),blood lipid(TG,TC,LDL-C,HDL-C),interleukin(IL-8,IL-1β,IL-6),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and malondialdehyde(MDA)were detected after drug delivery for 9 weeks.HE staining and oil red O staining were used to evaluate the pathological changes and steatosis of liver in mice.Western blot assay were used to detect the protein expressions of NF-κB p65 and HO-1 in liver tissues of mice.RESULTS EUP could reduce the body weight of db/db mice,reduce the levels of FBG,TG,TC,LDL-C,IL-8,IL-1β,IL-6,MDA,NF-κB p65 proteins and increase the levels of HDL-C and HO-1 proteins.EUP could alleviate pathological changes and steatosis of liver in db/db mice.CONCLUSION The obesity,hyperglycemia,hyperlipidemia and liver injury of db/db mice with type 2 diabetes are all improved by EUP,and the mechanism is related to the regulation of inflammatory response and oxidative stress.
作者
陈小娟
何福根
周迪夷
CHEN Xiao-juan;HE Fu-gen;ZHOU Di-yi(Cancer Hospital of the University of Chinese Academy of Sciences(Zhejiang Cancer Hospital),Hangzhou 310022,China;Hangzhou Red Cross Hospital,Hangzhou 310003,China)
出处
《中国药学杂志》
CAS
CSCD
北大核心
2020年第17期1433-1438,共6页
Chinese Pharmaceutical Journal
基金
国家自然科学基金项目资助(81703996)。
关键词
杜仲多糖
2型糖尿病
炎症反应
氧化应激
Eucommia ulmoides polysaccharide
type 2 diabetes
inflammatory response
oxidative stress
