摘要
目的：对木属植物中草本和木本两个自然群进行化学分类学的研究。方法：应用反相高效液相色谱法测定木属植物中４种化学成分［ｎｅｏｌｉｇｕｉｒｉｔｉｎ（Ｉ），５，８ｄｉｍｅｔｈｏｘｙｃｏｕｍａｒｉｎ（ＩＩ），ｉｓｏｌｉｑｕｒｉｔｏｓｉｄｅ（ＩＩ），８［（２″，３″）ｐｒｅｎｙｌ］４′ｍｅｔｈｏｘｙｆｌａｖｏｎｅ７ＯβＤｇｌｕｃｏｐｙｒａｎｏｓｙｌ（２→１）αＬｒｈａｍｎｏｐｙｒａｎｏｓｉｄｅ（ＩＶ）］，对本属１１种植物中该４种成分进行了定量分析。色谱柱固定相为ＳｕｐｅｌｃｏＳＩＬＬＣ１８；流动相为４０％甲醇—甲醇梯度洗脱，根据４种成分紫外吸收波长的不同而改变检测波长；流速１０ｍｌ·ｍｉｎ－１；４种成分的线性范围分别为（０５６００～００１７５）μｇ，（０３７６０～００１１８）μｇ，（０２８７５～０００９０）μｇ和（０３０９０～０００９７）μｇ，回收率分别为９８９２％，９８２７％，９４７８％和９７４１％。结果：木本自然群中香豆精成分含量较高。
AIM: To reveal the difference between the groups of herbal and woody in Aralia . METHODS: An accurate RP HPLC method with variable wavelength for the determination of neoliguiritin (I), 5,8 dimethoxycoumarin (II), isoliquritoside (III) and 8 [(2″,3″) prenyl] 4′ methoxy flavone 7 O β D glucopyranosyl (2→1) α L rhamnopyranoside (IV) in 11 species of Aralia has been developed. The analytical column used was Supelco SIL LC 18. The mobile phase was 40% MeOH to absolute MeOH. Gradient elution was employed. The flow rate was 1 0 ml·min -1 . From 0 00～8 30 min the elute was detected at 276 nm, 8 30～14 00 min at 343 nm, 14 00～20 00 min at 361 nm and 20 00～30 00 min at 272 nm. During the detection, the wavelength was changed to obtain high sensitivity and precision. The linear ranges of I～IV were 0 0175～0 5600 μg, 0 0118～0 3760 μg, 0 0090～0 2875 μg and 0 0097～0 3090 μg, respectively. The recoveries of I～IV were between 94 78% and 98 92%. RESULTS: The content of coumarin was higher in woody than in herbal. CONCLUSION: Coumarin is the specific constituent in the group of herbal, which is the difference from the woody.
出处
《药学学报》
CAS
CSCD
北大核心
1999年第1期46-48,共3页
Acta Pharmaceutica Sinica
基金
自然科学基金
