摘要
通过CRISPR/Cas9基因编辑技术,以粳稻品种‘沈农265’为遗传背景对水稻赤霉素2氧化酶基因OsGA2ox10进行编辑,获得了该基因编码序列翻译提前终止纯合突变体ga2ox10-1、ga2ox10-2和ga2ox10-3。经RT-PCR检测突变体中OsGA2ox10基因表达量相比野生型极显著降低。表达模式分析显示该基因在根、茎、叶、叶鞘、幼穗中均有表达,其中在幼穗中表达量最高。亚细胞定位结果显示OsGA2ox10与GFP融合蛋白定位于细胞质中。本实验的开展为进一步明确GA2ox10基因功能和该基因在赤霉素代谢失活调控网络中的作用提供了依据。
The rice gibberellin 2 oxidase gene OsGA2ox10 was edited by CRISPR/Cas9 in’Shennong 265’background.We obtain the homozygous mutants ga2ox10-1,ga2ox10-2 and ga2ox10-3 whose protein are early terminated.The expression level of OsGA2ox10 gene in mutants was significantly lower than that of wild type.Tissue expression pattern analysis showed that OsGA2ox10 was expressed in root,stem,leaf,leaf sheath and young panicle,and the expression level was highest in young panicles,Subcellular localization show that the OsGA2ox10-GFP fusion protein was localized to the cytoplasm.Therefore,our results provided a basis for clearing the function and role of OsGA2ox10 in the gibberellin metabolic inactivation regulation network.
作者
惠索祯
常俊楠
邱牡丹
李磊
王建龙
Hui Suozhen;Chang Junnan;Qiu Mudan;Li Lei;Wang Jianlong(College of Agronomy,Hunan Agricultural University,Changsha,410128;Hunan Jinjian Seed Industry Technology Co.,Ltd.,Changsha,410126)
出处
《分子植物育种》
CAS
CSCD
北大核心
2020年第18期6016-6024,共9页
Molecular Plant Breeding
基金
十三五国家重点研发计划(2016YFD0101105)
长沙市科技计划项目(kq1701160)共同资助。
