摘要
【目的】研究不同水分胁迫下新疆大叶苜蓿的生物量以及生理响应,分析二者之间的相关性,综合评价水分胁迫之间的抗旱差异.【方法】选取新疆大叶苜蓿为试验材料,采用盆栽试验法,对其进行3种水分处理(即充分供水、轻度胁迫和重度胁迫).在不同的水分胁迫下测定新疆大叶苜蓿的生物量分配、抗氧化酶活性及膜质过氧化物含量等指标并进行相关性分析.【结果】轻度水分胁迫下的总干物质积累最多,重度水分胁迫下的根冠比最大;叶片POD和SOD活性呈现出先升高后降低的变化趋势,轻度水分胁迫下保护酶的升高和下降表现最为显著;而CAT活性则呈现缓慢上升的变化趋势;MDA含量随着水分胁迫程度的加深和时间的延长持续增加;新疆大叶苜蓿生物量的变化、抗氧化酶活性及丙二醛含量部分指标间呈现出显著正(负)相关,部分指标间呈一定相关性但不显著.【结论】轻度水分胁迫既能满足新疆大叶苜蓿对水分的需求,促进植株生长,积累生物量,又能提高植物自身抗旱机能.
【Objective】Alfalfa(Medicago sativa cv.Xinjiang Daye)was selected as the experimental material to study the biomass and physiological response under different water stresses.The correlation between them was analyzed and the difference of drought resistance between water stresses was comprehensively evaluated.【Method】Three water treatments(full water supply,mild water stress and severe water stress)were designed for pot experiment.The biomass allocation,antioxidant enzyme activity and membrane peroxides content of alfalfa were determined.【Result】The total dry matter accumulation was the highest under mild water stress and the root-shoot ratio was the highest under severe water stress.The activity of POD and SOD in leaf increased first and then decreased,and the increase and decrease of protective enzymes were the most remarkable under mild water stress.CAT activity showed a trend of slowly increasing along with the stress.MDA content continued to increase with the water stress degree and time.【Conclusion】Mild water stress can not only meet the water demand of alfalfa,promote plant growth and biomass accumulation,but also improve the drought resistance.
作者
陈雅婷
罗永忠
申海宁
惠雅佞
CHEN Ya-ting;LUO Yong-zhong;SHEN Hai-ning;HUI Ya-ning(College of Forestry,Gansu Agricultural University,Lanzhou 730070,China)
出处
《甘肃农业大学学报》
CAS
CSCD
北大核心
2020年第4期128-136,共9页
Journal of Gansu Agricultural University
基金
国家自然科学基金项目(31560238)
甘肃省自然科学基金项目(17JR5RA145)
甘肃农业大学学科建设项目基金(GAU-XKJS-2018-111).
关键词
新疆大叶苜蓿
干旱胁迫
控水试验
生物量
保护酶
丙二醛
Medicago sativa cv.Xinjiang Daye
drought stress
water control experiment
biomass
protective enzyme
malondialdehyde
