摘要
本研究旨在探讨人参皂苷Rb1对佐剂性关节炎(AIA)大鼠的保护作用及机制.AIA大鼠腹腔注射给药Rb1,连续给药14 d,观察大鼠足肿胀、关节病理及血清炎症细胞因子水平.并通过体外实验检测Rb1对LPS诱导的RAW264.7细胞分泌NO、TNF-α及IL-6水平的影响,进一步评估Rb1的抗炎作用.Western blot检测关节滑膜及RAW264.7细胞中NF-κB信号通路相关蛋白表达,阐明Rb1抗RA作用机制.结果显示,Rb1显著减轻AIA大鼠足肿胀和关节破坏,降低血清TNF-α、IL-1β及IL-6水平并抑制LPS诱导的RAW264.7细胞分泌NO、TNF-α及IL-6水平.Western blot显示Rb1下调关节滑膜p-IκBα及p-p65的表达,上调RAW264.7细胞IκBα的表达.以上结果提示,Rb1对AIA大鼠具有保护作用,与抑制关节滑膜中NF-κB信号通路的激活有关.
A novel approach is presented to investigate the anti-RA effect and underlying mechanism of Rb1 in adjuvant-induced arthritis(AIA)rats.AIA rats receive Rb1 intraperitoneally for 14 days,then the joint swelling,histopathology and inflammatory cytokines are evaluated.To further evaluate the anti-inflammatory effects,NO,TNF-α,and IL-6 levels are measured in LPS-induced RAW264.7 cells.Meanwhile,western blot is used to detect the expression of NF-κB signaling pathway proteins in synovial membranes and RAW264.7 cells.The results show that Rb1 significantly relieves joint swelling and injuries,reduces serum TNF-α,IL-1β and IL-6 levels in AIA rats,and inhibits NO,TNF-α and IL-6 levels in LPS-induced RAW264.7 cells.Moreover,Rb1 significantly reduces the protein expressions of p-IκBα and p-p65 in synovial membranes,while it remarkably enhances the protein expressions of IκBαin LPS-induces RAW264.7 cells.Therefore,Rb1 exertes therapeutic effects on AIA rats through inhibiting the activation of the NF-κB.
作者
王美灵
黄亚楠
李敏敏
王艳芳
张潇文
张雷明
WANG Mei-ling;HUANG Ya-nan;LI Min-min;WANG Yan-fang;ZHANG Xiao-wen;ZHANG Lei-ming(School of Pharmacy,Key Laboratory of Molecular Pharmacology and Drug Evaluation,Ministry of Education,Yantai University,Yantai 264005,China)
出处
《烟台大学学报（自然科学与工程版）》
CAS
2020年第3期307-313,共7页
Journal of Yantai University(Natural Science and Engineering Edition)
基金
山东省自然科学基金资助项目(ZR2017MH068)
烟台市重点研发计划项目(2019XDHZ109).
