摘要
[目的]高效利用纤维素资源,保护生态环境。[方法]利用刚果红染色法从土壤中筛选出高效纤维素降解菌且对其进行形态学和分子学鉴定。[结果]菌株Ⅰ的纤维素酶活力0.0053 U/mL,与Bacillus pumilus strain 35的亲缘关系为99%,命名为Bacillus pumilus WL-1,为革兰氏阳性菌。菌株Ⅱ的纤维素酶活力0.0059 U/mL,与Bacillus pumilus strain BAB-1322的亲缘关系为99%,命名为Bacillus pumilus WL-2,为革兰氏阳性菌。[结论]筛选的纤维素分解菌具有较强的纤维降解能力,可有效提高纤维素资源的开发利用。
[Objective]To make efficient use of cellulose resources and protect the ecological environment.[Method]Congo red staining was used to screen high-efficient cellulose-degrading bacteria from soil and identify them morphologically and molecular.[Result]The results showed that the cellulase activity of strain I was 0.0053 U/mL and its relationship with Bacillus pumilus strain 35 was 99%.The strain I was named Bacillus pumilus WL-1,which was gram-positive.The cellulase activity of strain II was 0.0059 U/mL and 99%of its relatives with Bacillus pumilus strain BAB-1322.The strain II was named Bacillus pumilus WL-2 and was gram-positive.[Conclusion]The cellulose-decomposing bacteria screened in this study have strong cellulose-degrading ability and can effectively improve the development and utilization of cellulose resources.
作者
张海艳
王文磊
韩锰
ZHANG Hai-yan;WANG Wen-lei;HAN Meng(Department of Life Science,Luoyang Normal University,Luoyang,Henan 471934)
出处
《安徽农业科学》
CAS
2020年第15期1-3,8,共4页
Journal of Anhui Agricultural Sciences
基金
河南省高等学校重点科研项目(19A180023)。
关键词
土壤
纤维素分解菌
筛选鉴定
纤维素酶活力
Soil
Cellulose decomposing bacteria
Screening and identification
Cellulase activity
