摘要
以滤纸平板和羧甲基纤维素钠培养基为基础培养基,从采集的样品中筛选出具有分解纤维素能力的38株菌株。采用纤维素刚果红培养基进行粗选,得到10株透明圈较大的菌株。将这10株菌株进行液体发酵培养,测定其酶活力,得到4株分解纤维素能力较强的菌株。对这4株菌株进行碳源、温度、pH值的适应性研究。结果发现,真菌3和真菌6的适应性都比较好。
Filter paper and carboxylic methyl fiber atrium were used as media to isolate and screen cellulose-decomposing microorganisms, and 38 strains were isolated from samples of soil and compost prepared from chicken manure, swine manure and cow manure. Of the isolated 38 strains, 10 strains showed a great capacity in cellulose decomposing, indicat ed by large and distinct red circles around the colonies in Congo red agar medium. Four strains of fungi were obtained with highest cellulase activity using liquid medium. Study on the adaptability to carbon sources, temperature and pH showed that two strains have a great potential to be used as composting inoculums.
出处
《科技通报》
2005年第2期236-241,共6页
Bulletin of Science and Technology
基金
嘉兴市-浙江大学合作项目国家人事部留学回国人员择优项目国家教委留学回国人员基金
关键词
纤维素分解菌
纤维素
分离筛选
cellulose-decomposing microorganism
cellulose
isolation and screening
