摘要
利用分光光度法以对硝基月桂酸酯为底物溶液对诺维信脂肪酶435进行活性表征。结果表明:诺维信脂肪酶435作用的最适温度为40~60℃,最适pH为8.00~11.00,Ba^2+、K^+,Tween-20,EDTA和PMSF都可以适当提高酶的活性,而去污剂Triton X-100以及Cu^2+、Ca^2+、Zn^2+则会使酶活性有不同程度的降低。另外反复使用10次后诺维信脂肪酶的活性仍然在80%左右。该研究可以为该酶的在生物柴油制备等应用提供一定的数据参考。
The activity of Novozymes lipase 435 was characterized by spectrophotometry with p-nitrolaurate as substrate solution.The results showed that the optimum temperature of Novozymes lipase 435 was 40-60℃,the optimum pH was 8.00-11.00.Ba^2+,K^+,Tween-20,EDTA and PMSF all could properly increase the activity of the enzyme,while the detergent Triton X-100 and Cu^2+,Ca^2+,Zn^2+ could reduce the activity of the enzyme to varying degrees.In addition,the activity of Novozymes lipase 435 was still about 80%after repeated use for 10 times.This study can provide some data reference for the application of the enzyme in biodiesel preparation and so on.
作者
田志美
宋崇富
卢若雨
王标
薄连震
刘锐锐
蒋雪月
金晓艳
TIAN Zhimei;SONG Chongfu;LU Ruoyu;WANG Biao;BO Lianzhen;LIU Ruirui;JIANG Xueyue;JIN Xiaoyan(School of Chemistry&Materials Engineering,Fuyang Normal University,Fuyang Anhui 236037,China;Anhui Hengyu Environmental Protection Equipment Manufacturing Limited Company,Fuyang Anhui 236626,China)
出处
《阜阳师范大学学报（自然科学版）》
2020年第3期24-29,共6页
Journal of Fuyang Normal University:Natural Science
基金
阜阳市政府-阜阳师范学院横向合作项目(XDHX201719)资助。
关键词
诺维信脂肪酶435
分光光度法
活性表征
条件探究
Novozymes Lipase 435
spectrophotometry
activity characterization
conditional investigation
