摘要
目的完善滋阴补肾丸的质量标准。方法采用薄层色谱法对滋阴补肾丸处方中的人参、黄芪、五味子、山药、山茱萸进行定性鉴别,采用高效液相色谱法测定黄芪中黄芪甲苷的含量,用蒸发光散射检测器检测,雾化器温度为99.3℃,气体为氮气,流速为2.7 L/min,采用外标二点法对数方程计算含量。结果薄层色谱图斑点清晰,阴性对照无干扰;黄芪甲苷进样量在11.31~67.86μg,r=0.9990(n=6)范围内与峰面积线性关系良好;平均加样回收率为97.64%,RSD为1.70%(n=9)。结论该方法专属性强、重复性好,可为建立滋阴补肾丸的质量标准提供参考。
Objective To improve the quality standard of Ziyin Bushen Pills.Methods Qualitative identification of ginseng,schisandra,yam,and cornus in the prescription of Ziyin Bushen Pills was conducted by thin layer chromatography(TLC);determination of astragalosideⅣin astragalus was conducted by high performance liquid chromatography(HPLC).Detection was done by evaporative light scattering detector;the atomizer temperature was 99.3℃,the gas was nitrogen and the flow rate was 2.7 L/min;the external standard two-point method was used to calculate the content by the logarithmic equation.Results TLC showed clear spots,with no interference with the negative contrast;the injection volume of astragalosideⅣin the range of 11.31-67.86μg had a good linear relationship with the peak area(r=0.9990),and the average sample recovery was 97.64%,the RSD was 1.70%(n=9).Conclusion This method is highly specific and reproducible,which can provide a reference for establishing the quality standard of Ziyin Bushen Pills.
作者
汪爱霞
孙芸
郭文宾
WANG Aixia;SUN Yun;GUO Wenbin(Gansu Hexi Pharmaceutical Co.Ltd.,Zhangye,Gansu,China 734000;Gansu Zhangye Foodand Drug Inspectionand Testing Center,Zhangye,Gansu,China 734000)
出处
《中国药业》
CAS
2020年第15期51-54,共4页
China Pharmaceuticals
关键词
滋阴补肾丸
薄层色谱法
高效液相色谱-蒸发光散射检测器法
黄芪甲苷
质量标准
Ziyin Bushen Pills
thin layer chromatography
high performance liquid chromatography-evaporative light scattering detection
astragalosideⅣ
quality standard
