摘要
目的:探讨荭草苷(Ori)对APP/PS1转基因小鼠海马神经元的神经保护作用。方法:将10月龄APP/PS1转基因小鼠随机分为模型组(Tg组)、Ori低剂量组(Tg/Ori-L组)和Ori高剂量组(Tg/Ori-H组),将同月龄C57BL/6J小鼠作为对照组(NT组)。采用Morris水迷宫实验检测各组小鼠空间学习、记忆能力;采用Western blot法检测各组小鼠海马区脑源性神经生长因子(BDNF)、酪氨酸激酶B(TrkB)受体的蛋白表达;采用ELISA法检测β-淀粉样蛋白1-42(Aβ1-42)水平。结果:与NT组比较,Tg组小鼠寻找平台的逃避潜伏期延长及穿越目的象限的次数减少(P<0.05),海马区BDNF、TrkB受体表达降低(P<0.05),Aβ1-42表达升高(P<0.05)。与Tg组比较,Tg/Ori-L组和Tg/Ori-H组各指标均显著改善(P<0.05),但Tg/Ori-L组与Tg/Ori-H组间比较无统计学差异(P>0.05)。结论:Ori可以改善APP/PS1小鼠空间学习记忆能力,其机制可能与上调海马神区经元BDNF及TrkB受体表达、减少脑内Aβ沉积有关。
Objective:To explore neuroprotective mechanism of orientin(Ori)on hippocampal neurons of APP/PS1 transgenic mice.Methods:10 months old APP/PS1 transgenic mice were randomly divided into three groups:model group(Tg),Ori low-dose group(Tg/Ori-L)and Ori high-dose group(Tg/Ori-H).C57 BL/6 J mice of the same age were utilized as control group(NT group).The spatial learning and memory ability of mice in each group were detected by Morris water maze test.The protein expression of BDNF and TrkB receptor in hippocampus of mice in each group were detected by Western blot.The level of Aβ1-42 was detected by ELISA.Results:Compared with NT group,the escape latency of TG group was prolonged and the times of crossing the target quadrant was decreased(P<0.05),the expression of BDNF and TrkB receptor in hippocampus was decreased(P<0.05),and the expression of Aβ1-42 was increased(P<0.05).Compared with TG group,TG/Ori-L group and TG/Ori-H group showed significant improvement(P<0.05),but there was no significant difference between TG/Ori-L group and TG/Ori-H group(P>0.05).Conclusion:Ori can improve the spatial learning and memory ability of APP/PS1 mice,and its mechanism may be related to the up regulation of BDNF and TrkB receptor expression in hippocampal neurons and the reduction of Aβdeposition in brain.
作者
张泽
韩锟
贾宁
ZHANG Ze;HAN Kun;JIA Ning(Department of Neurology,the First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121001)
出处
《陕西医学杂志》
CAS
2020年第5期534-537,共4页
Shaanxi Medical Journal
基金
辽宁省自然科学基金指导计划项目(2019-ZD-0616)
辽宁省自然科学基金计划项目(2013022028)。
