摘要
为了明确莼菜中锌蛋白的分布及氨基酸组成,以富硒高锌土壤中种植的莼菜为材料,对其幼叶蛋白质进行分离提取和分析。结果表明:莼菜中蛋白质主要类型为弱碱性蛋白质,比例为44.79%,但其锌含量相对较低,水溶性蛋白比例只有15.43%,但其锌含量却是弱碱性蛋白质中的2.17倍,表明大部分锌离子是与水溶性蛋白结合的;通过逐级盐析从水溶性蛋白中获得5个馏分,进一步分析各馏分的蛋白质含量及锌含量,获得WSⅡ和WSⅤ这2个高锌含量的蛋白质馏分,电泳分析显示这2个馏分分别由一个或一系列相同亚基组成;氨基酸组分分析显示,WSⅡ和WSⅤ中甘氨酸、硒代半胱氨酸、组氨酸和赖氨酸含量较高。
Brasenia schreberi planted in selenium-rich and high-zinc soil was used as the target material in this study.The proteins in Brasenia schreberi young leaves were separated and analyzed for further clarification of zinc protein distribution and amino acid composition in the genus.The results show that the main type of proteins in Brasenia schreberi is weak alkaline protein,accounting for 44.79%,but its zinc content is relatively low;the proportion of water-soluble protein is only 15.43%,but its zinc content is 2.17 times that of weak alkaline protein,which indicates that most of the zinc ions are combined with water-soluble protein.Five fractions from watersoluble protein were obtained by stepwise salting out,then the protein content and zinc content of each fraction were analyzed,finally two protein fractions of high zinc content WSⅡand WSⅤwere obtained.Electrophoresis analysis shows that these two fractions are composed of one or a series of the same subunits.Amino acid composition analysis shows that the contents of glycine,selenocysteine,histidine and lysine are high in WSⅡand WSⅤ.
作者
肖鹜轩
王昆仑
周海燕
XIAO Wu-xuan;WANG Kun-lun;ZHOU Hai-yan(Hunan Institute of Nuclear Agriculture Science and Space Breeding,Hunan Academy of Agricultural Sciences,Changsha 410125,PRC;College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410128,PRC)
出处
《湖南农业科学》
2020年第4期64-68,共5页
Hunan Agricultural Sciences
基金
湖南省战略性新兴产业科技攻关与重大成果转化项目(2019GK4018)
湖南农业大学“双一流”建设项目(SYL201802002)。
关键词
莼菜
锌蛋白
分离
氨基酸组成
Brasenia schreberi
zinc protein
separation
amino acid composition
