摘要
相干拉曼散射显微技术作为一种新型的成像技术,具有无标记、高特异性、非侵入等优点,已被广泛用于化学结构及物质成分分析。近年来,光子学、生物医学和显微成像技术等领域的相互交叉和融合发展,极大地推动了相干拉曼散射显微成像技术在生物医学领域的应用。简要介绍了相干拉曼散射显微成像的基本原理、分类、系统构成,同时概述了相干拉曼散射显微成像技术近年来在生物医学领域的应用,包括检测、脂类分析和蛋白质构象变化等,最后对其未来发展进行了展望。
As a new imaging technique,coherent Raman scattering(CRS)microscopy has been widely used in chemical structure and composition analysis because of its advantages of label-free,high specificity,and on-invasion.In recent years,the mutual cross-over and integrated development of photonics,biomedicine,and microscopic imaging technology has greatly promoted the application of CRS microscopy in biomedicine.This paper briefly introduces the basic principle of CRS microscopy and its classification,then explains the most widely used implementation of CRS,and summarizes the recent applications of CRS microscopic imaging in biomedicine,including detection,lipid analysis,and protein conformation change.Finally,the future development of CRS is discussed.
作者
李姿霖
李少伟
张思鹭
沈炳林
屈军乐
刘丽炜
Li Zilin;Li Shaowei;Zhang Silu;Shen Binglin;Qu Junle;Liu Liwei(Key Laboratory of Optoelectronics Devices and Systems of Guangdong Province and Ministry of Education,College of Physics and Optoelectronic Engineering,Shenzhen University,Shenzhen,Guangdong 518060,China)
出处
《中国激光》
EI
CAS
CSCD
北大核心
2020年第2期73-83,共11页
Chinese Journal of Lasers
基金
国家自然科学基金(61525503,61620106016,61835009)
广东省高等学校科技创新(重点)项目(2015KGJHZ002,2016KCXTD007)
广东省自然科学基金创新研究团队项目(2014A030312008)
深圳市自由探索项目(JCYJ20180305124902165)。
关键词
生物医学
相干拉曼散射显微技术
相干反斯托克斯拉曼散射
无标记成像
受激拉曼散射成像
biomedicine
coherent Raman scattering microscopy technique
coherent anti-stokes Raman scattering
label-free imaging
stimulated Raman scattering imaging
