摘要
目的针对目前对人体总铂的浓度检测方法不一,缺乏既定标准且检测成本较高的现象,探索国内较常用的仪器-原子吸收分光光度仪检测肿瘤患者用药后血清中总铂浓度的方法。方法使用石墨炉原子吸收分光光度仪(GFAAS-650)检测肿瘤患者用药后的血清总铂浓度,以吸光度大小、峰型作为判断标准,判定适合的温度程序,通过回收率验证所得温度程序的适用性,并确定前处理过程是否需要使用基体改进剂。结果获得以1150℃为灰化温度、2700℃为原子化温度的石墨炉升温程序为最佳程序,检测结果回收率为94.77%,前处理过程不需使用基体改进剂,方法检出限为31.16μg·L-1。结论本方法前处理过程简便,准确度及灵敏度高,经济成本低,适合大部分医疗或科研单位进行患者总铂含量监测。
Objective To obtain a method of determination on serum platinum from tumor patients after medication by atomic absorption spectrophotometer,directing at the inconsistent Methods and high cost on the detection of total platinum concentration in human body.Methods To determinate platinum concentration of cancer patient serum by GFAAS-650 with the methods of manufacturers recommending and adjusting.The valuations of temperature programs contained absorption,peak and recovery.Absorption and peak were also to use to estimate whether to add matrix modifier or nitric acid only.Results The program with an ashing temperature of 1150℃and atomization temperature of 2700℃was best one for the detection of serum platinum,while its recovery was 94.77%without any matrix modifier and the limit of detection was 31.16μg·L-1.Digestion without matrix modifier for the sample pretreatment was the proper method.Conclusion We got a convenient,accurate and sensitive method on the determination of total platinum in patients for most medical and scientific institutes with lower cost.
作者
张浴玲
郭岱年
陈晨
方翎
ZHANG Yuling;GUO Dainian;CHEN Chen;FANG Ling(Laboratory of Environmental Medicine and Developmental Toxicology,Shantou University Medical College,Shantou 515041,China;Pharmacy Intravenous Admixture Service,Cancer Hospital of Shantou University Medical College,Shantou 515041,China)
出处
《药学研究》
CAS
2020年第4期193-197,共5页
Journal of Pharmaceutical Research
基金
汕头市科技计划项目(No.2016-6)
广东省科技计划项目(No.2014A020212505)。
关键词
铂
血清
原子吸收
检测
Platinum
Serum
Atomic absorption
Determination
