摘要
目的探究结直肠癌(CRC)患者术前血清CA19-9和CCL-21表达水平与其临床病理特征及预后的相关性。方法选择2012年1月至2013年6月本院收治的156例CRC患者为研究组,另选择同期45例结直肠组织正常者为对照组。以酶联免疫吸附法(ELISA)测定血清CA19-9水平,双抗夹心ELISA测定CCL-21表达水平,分析两者表达与患者病理特征和预后的关系。结果研究组血清CA19-9和CCL-21水平均显著高于对照组(P<0.05)。肿瘤病灶分化程度低、TNM分期Ⅲ期、有淋巴结或肝转移患者的CA19-9阳性率及CCL-21高水平表达率均高于分化程度高、TNM分期Ⅱ期、无淋巴结及肝转移患者(P<0.05)。Kaplan-Meier法分析显示,CA19-9阳性表达与CCL-21水平高表达者累计生存时间明显短于CA19-9阴性表达和CCL-21水平低表达者(P<0.05)。结论CA19-9和CCL-21与CRC瘤体分化、TNM分期和淋巴结或肝转移有关,其高表达提示患者预后不良。
Objective To explore the correlation between preoperative serum CA19-9 and CCL-21 expression levels and clinicopathological features and prognosis in patients with colorectal cancer(CRC).Methods A total of 156 CRC patients admitted in our hospital from January 2012 to June 2013 were selected as study group,and 45 patients with normal colorectal tissue in the same period were selected as control group.Serum CA19-9 level was measured by enzyme-linked immunosorbent assay(ELISA),and CCL-21 expression level was measured by double antibody sandwich ELISA,and the relationship between the expression levels of CA19-9 and CCL-21 and the pathological features and prognosis of the patients were analyzed.Results The levels of serum CA19-9 and CCL-21 in the study group were significantly higher than those in the control group(P<0.05).The positive rate of CA19-9 and high expression rate of CCL-21 in patients with low differentiation of tumor focus,TNM stageⅢ,lymph node metastasis and liver metastasis were higher than those in patients with high differentiation of tumor focus,TNM stageⅡ,no lymph node metastasis and no liver metastasis(P<0.05).Kaplan-meier analysis showed that the cumulative survival time of patients with positive expression of CA19-9 and high expression of CCL-21 were significantly shorter than those in patients with negative expression of CA19-9 and low expression of CCL-21(P<0.05).Conclusion CA19-9 and CCL-21 are related to tumor differentiation,TNM staging,and lymphaden or liver metastasis of CRC.The high expression of CA19-9 and CCL-21 indicates poor prognosis.
作者
孔延龙
李云龙
项红军
李纪鹏
胡氏亮
KONG Yan-long;LI Yun-long;XIANG Hong-jun;LI Ji-peng;HU Shi-liang(No.1 General Surgery Department,Xi'an Daxing Hospital,Xi'an 710003,China)
出处
《临床医学研究与实践》
2020年第9期11-13,共3页
Clinical Research and Practice
基金
国家自然科学基金项目(No.81672751)。
