摘要
该研究利用TNF-α诱导建立肿瘤细胞体外侵袭模型,为进一步的研究提供基础。利用20 ng/mL TNF-α刺激结肠癌细胞HCT116 7 d后,使用流式细胞术检测HCT116细胞的CCR7和CXCR4受体表达量的变化,并利用Transwell小室检测TNF-α刺激对CCL21、SDF-1介导的细胞迁移与侵袭能力的影响。实验结果显示,20 ng/mL TNF-α刺激7 d后的HCT116细胞的CCR7和CXCR4表达量均显著增加,侵袭能力也增强,且使CCL21、SDF-1介导的细胞迁移与侵袭能力显著增强。结果说明了本实验利用TNF-α诱导HCT116细胞,成功建立了HCT116的体外侵袭模型,为接下来的研究提供了细胞模型基础,也为进一步的药物筛选提供了基础。
This research focused on establishing an in vitro HCTI 16 cells invasion model for drug screen ing. In this study, HCTll6 human colon cancer ceils were treated by 20 ng/mL TNF-α for 7 days, then the expres sion of CCR7 and CXCR4 receptors were detected by flow cytometry. The cell invasion ability of CCL21 and SDF- 1 was further evaluated by transwell chamber. After stimulated by 20 ng/mL TNF-α for 7 days, the expression of CCR7 and C XCR4 increased significantly which facilitated the migration of HCT 116. Furthermore, the CCL21 and SDF-1 could enhance the cell invasiveness. The results of this study demonstrate that the invasiveness of HCTII 6 can be enhanced by TNF-a, so that it can be used to establish an invasion model in vitro for drug screening.
出处
《中国细胞生物学学报》
CAS
CSCD
北大核心
2012年第10期1004-1009,共6页
Chinese Journal of Cell Biology
基金
国家重点基础研究发展计划(973计划)(No.2011CB9358003)
国家大学生创新性实验计划(No.101055836)
国家自然科学基金(No.30873032)资助项目~~
