摘要
采用火焰分光光度法对从烟草根际土壤中分离到细菌ZMD02的解钾活性进行了测定,结果表明,在28℃、180 r/min条件下发酵培养7 d后,菌株ZMD02发酵液中水溶性速效钾含量达1.5μg/mL,故将ZMD02定性为硅酸盐菌株。对ZMD02的生长特性研究表明,ZMD02对不同糖源的发酵产气能力表现为葡萄糖>麦芽糖>乳糖>直链淀粉>支链淀粉;对碳同化能力表现为麦芽糖>葡萄糖>乳糖>直链淀粉>支链淀粉;ZMD02对不同氮源的同化能力表现为酵母膏>蛋白胨>硫酸铵>硝酸钾>尿素;ZMD02的最适生长温度为30℃,最适pH为7。ZMD02菌株可作为烟草生物钾肥开发的备用菌株。
The potassium activity of bacteria ZMD02 isolated from tobacco rhizosphere soil was determined by flame spectrophotometry.The results showed that after 7 days of fermentation at 28℃and 180 r/min,the content of water-soluble rapidly available potassium in ZMD02 fermentation broth reached 1.5μg/mL.Therefore,ZMD02 was characterized as silicate strain.The growth characteristics of ZMD02 showed that the order of fermentation capacity of ZMD02 to different sugar sources was glucose>maltose>lactose>amylose>amylose.The order of carbon assimilation ability was maltose>glucose>lactose>amylose>amylose.The order of assimilation ability of ZMD02 to different nitrogen sources was yeast extract>peptone>ammonium sulfate>nitrate>urea.The optimum growth temperature of ZMD02 was 30℃and the optimum pH was 7.ZMD02 strain could be used as an alternative strain for the development of biopotash fertilizer in tobacco.
作者
吴俊林
郭传滨
贾真
郝浩浩
许晓敬
杨立均
孙会忠
WU Jun-lin;GUO Chuan-bin;JIA Zhen(Zhumadian Branch of Henan Provincial Tobacco Company,Zhumadian,Henan 463000)
出处
《安徽农业科学》
CAS
2020年第7期170-171,175,共3页
Journal of Anhui Agricultural Sciences
基金
河南省烟草公司驻马店市公司项目“植烟土壤的微生物定向调控保育技术研究”
河南省高等学校重点科研项目(16A180003)。
关键词
烟草
解钾菌
生长特性
Tobacco
Silicate bacteria
Growth characteristics
