摘要
六价铬C(rⅥ)具有较强溶解性、迁移能力、生物相容性和热稳定性,可被人体吸收,且毒性很大。铬在工业中的广泛使用已造成严重的环境污染。从土壤中分离的抗铬细菌可以用于去除受污染环境中的六价铬。自重庆市秀山县某矿厂铬渣堆放场周围的土样中分离筛选出15株抗铬菌株,其中菌株C-10对C(rⅥ)有较高抗性和去除率,C(rⅥ)对菌株C-10的最低抑菌浓度为12 800 mg/L;在C(rⅥ)浓度为200 mg/L时,C-10对六价铬的去除率达到70.4%。对该菌株进行形态学观察、生理生化特征分析以及16S rDNA序列分析,菌株C-10被鉴定为一株纤维化纤维菌(Cellulosimicrobium cellulans)。研究pH和温度在铬环境下对抗铬菌株C-10生长的影响,发现在C(rⅥ)浓度为200mg/L时,C-10菌株在pH 8.0、温度30℃条件下生长状况最好。C-10菌株为微生物修复C(rⅥ)污染提供了可能的菌种资源。
Hexavalent chromium Cr （Ⅵ） which is soluble, transportable, biocompatible, thermostable, poisonous can be absorbed into human body. Extensive use of chromium in various industrial applications has caused substantial environmental contamination, and chromium-resistant bacteria isolated from soils can be used to remove toxic Cr（ Ⅵ） from contaminated environments. 15 Cr（ Ⅵ ） resistant strains were isolated from the soils contaminated with dichromate, in which the strain C-10 exhibited a higher resistance to Cr（ Ⅵ ） and the highest removal rate of Cr（ VI ）, with minimum inhibition concentration （MIC） of C-10 to Cr（Ⅵ） as 12 800 mg/L. Cr（Ⅵ） removal rate of C-10 was found to be 70.4% at an initial concentration of 200 mg/L. According to morphological, physiological, biochemical characteristics and 16S rDNA sequence, the strain C-10 was identified as Cellulosimicrobium cellulans. Effects of pH and temperature on C-10 were studied, and results showed that C-10 grew best when pH was 8.0 and temperature was 30 ~C at a concentration of 200 mg/L of Cr（ VI ）.
出处
《环境科学与技术》
CAS
CSCD
北大核心
2013年第9期36-40,共5页
Environmental Science & Technology
基金
四川省教育厅重点项目(10ZA006)
关键词
C(rⅥ)
抗铬细菌
纤维化纤维菌
分离
生长条件
Cr（ VI ）
Cr（ VI ）resistant bacterium
CeUulosimicrobium cellulans
isolation
growth condition
