摘要
目的了解北京及其周边地区儿童手足口病(HFMD)的流行特点及病原学特征。方法采集北京及其周边地区2017—2018年儿童HFMD病例咽拭子样本,使用肠道病毒(EV)通用、EV71及柯萨奇病毒A16型(CA16)三通道Real-time PCR的方法进行肠道病毒核酸检测,对非EV71非CA16的EV阳性样本采用RT-PCR法扩增VP1基因片段,通过BLAST比对进一步进行型别鉴定。结果2017—2018年共纳入HFMD患者939例,包括重症病例2例;男女性别比为1.54∶1;3岁以下儿童占46.96%,5岁以下儿童占75.19%。EV总阳性检出率为89.24%(838/939),其中2017年、2018年分别为87.44%(181/207)和89.75%(657/732);2017年主要为CA6(47.51%)、EV71(16.02%)、CA16(7.73%)和未分型EV(27.62%);2018年包括CA6(46.88%)、CA16(14.31%)和未分型EV(36.99%)。结论2017—2018年,引起北京及其周边地区儿童HFMD的主要病原体是CA16,EV71的检出率显著下降,因此需要加强HFMD病原体中CA6等非EV71非CA16肠道病毒的监测工作。
Objective To investigate the epidemic features and etiological characteristics of hand,foot and mouth disease(HFMD)in children in Beijing and surrounding areas from 2017 to 2018.Methods The throat swabs of HFMD cases were collected in Beijing and surrounding area from 2017 to 2018.Real-time PCR was used to detected general enterovirus(EV)-EV71/CA16(Coxsackievirus A16).The VP1 gene sequences of non-EV71 and non-CA16 enterovirus positive samples were amplified by RT-PCR and the amplicons were sequenced.Nucleotide BLAST was used to identify the species of enterovirus.Results A total of 939 HFMD patients were enrolled in this study,including 2 severe cases.The ratio of male to female was 1.54∶1.The majority of cases were younger than five year sold,which accounted for 75.19%,and the children under three years old accounted for 46.96%.The detection rate of EV was 89.24%(838/939),while the rates were 87.44%(181/207)and 89.75%(657/732)in 2017 and 2018,respectively.In 2017,the main EVs were CA6(47.51%),EV71(16.02%),CA16(7.73%)and non-type EV(27.62%);and in 2018,the main EVs were CA6(46.88%),CA16(14.31%)and non-type EV(36.99%).Conclusions CA6 is the main pathogen of HFMD in children in Beijing and surrounding areas from 2017 to 2018,and the detection rate of EV71 was decreased.Therefore,more attentions should be paid for the surveillance for other EV besides EV71 and CA16.
作者
张萌
段亚丽
王巍
朱云
陈祥鹏
谢正德
Zhang Meng;Duan Yali;Wang Wei;Zhu Yun;Chen Xiangpeng;Xie Zhengde(Research Unit of Critical Infection in Children,Chinese Academy of Medical Sciences,Key Laboratory of Major Diseases in Children,Ministry of Education,Beijing Key Laboratory of Pediatric Respiratory Infection Diseases,Virology Laboratory,Beijing Pediatric Research Institute,Beijing Children’s Hospital,Capital Medical University,National Center for Children’s Health,Beijing 100045,China)
出处
《国际流行病学传染病学杂志》
CAS
2020年第1期21-24,共4页
International Journal of Epidemiology and Infectious Disease
基金
国家科技重大专项(2017ZX10104001-005-010,2018ZX10201002-003-009)
国家自然科学基金(81971922)
北京市自然科学基金(7184208)
首都医科大学基础临床科研合作课题(17JL11)。
关键词
手足口病
流行病学
病原学
柯萨奇病毒A组6型
Hand
foot and mouth disease
Epidemiological characteristics
Etiology
Coxsackievirus A6
