摘要
为了探究金针菇与杏鲍菇混合发酵的条件,从而更好地利用金针菇与杏鲍菇的营养,采用单因素试验法以胞外多糖产生量和菌丝体生物量为指标,探究金针菇和杏鲍菇混合发酵培养时的最适碳源、最适氮源、最适无机盐、最适温度、最适转速,然后通过正交试验法找出碳源、氮源和无机盐的最适配比。结果表明,金针菇和杏鲍菇混合发酵液培养的优化条件为在基本液体培养基中加入蛋白胨2%,麦芽糖2%,FeSO 40.5%,KH 2 PO 43.6%,最适温度26℃,最适转速110 r/min。
In order to explore the conditions for the mixed fermentation of Flammulina velutiper and Pleurotus eryngii,so as to make better use of the nutrition of Flammulina velutiper and Pleurotus eryngii,the single factor experiment method is used to explore the optimal carbon source,nitrogen source,inorganic salt,temperature and rotation speed for the mixed fermentation of Flammulina velutiper and Pleurotus eryngii with exopolysaccharide production and mycelia biomass as the indicators,and then the orthogonal experiment method is used to find out the best ratio of carbon source,nitrogen source and inorganic salt.The results show that the optimum conditions of the mixed fermentation broth culture are:peptone of 2%,maltose of 2%,FeSO 4 of 0.5%,KH 2 PO 4 of 3.6%,the optimum temperature of 26℃,and the optimum rotation speed of 110 r/min.
作者
罗青
杨玉珍
王国霞
陈刚
LUO Qing;YANG Yu-zhen;WANG Guo-xia;CHEN Gang(College of Life Sciences,Zhengzhou Normal University,Zhengzhou 450044,China)
出处
《中国调味品》
CAS
北大核心
2020年第3期129-136,共8页
China Condiment
基金
河南省2017科技攻关项目(172102110138)
关键词
金针菇
杏鲍菇
混合发酵
多糖
正交试验
Flammulina velutiper
Pleurotus eryngii
mixed fermentation
polysaccharide
orthogonal experiment
