摘要
目的基于系统药理学方法探究麻黄细辛附子汤治疗缓慢型心律失常的作用机制。方法利用中药系统药理学分析平台(TCMSP)和PharmMapper数据库筛选出麻黄细辛附子汤的有效成分和作用靶点,并构建成分-靶点网络,然后利用OMIM、GeneCards、CTD数据库筛选缓慢型心律失常的相关靶点,通过Draw Venn Diagram网站进行交集比对,获取麻黄细辛附子汤作用于缓慢型心律失常的相关靶点;然后利用DAVID数据库对交集靶点进行基因本体(GO)功能富集分析和KEGG通路富集分析;利用Cytoscape进行网络靶点特性分析,并在Systemsdock数据库进行系统分子对接。结果筛选出了51个满足条件的药物有效成分及其263个作用靶点,566个缓慢型心律失常相关的疾病靶点,交集靶点共14个。GO分析得到63个相关生物过程,6项分子功能,10类细胞组成;KEGG通路富集筛选得到5条重要的信号通路;各分子与靶点之间有较好的结合活性。结论麻黄细辛附子汤可能是通过唾腺分泌、心肌细胞的肾上腺素能、cGMP-pkg信号通路、钙信号通路、cAMP信号通路来发挥对缓慢型心律失常的治疗作用。
Objective To explore the mechanism of Ephedra Asarum Aconite(EAA) decoction in the treatment of bradyarrhythmia based on systematic pharmacology. Method The effective components and action targets of EAA decoction were screened by TCMSP and PharmMapper database, and the network of components and targets was constructed.Then, the related targets of bradyarrhythmia were screened by OMIM, GeneCards, CTD database. Through the intersection comparison of Draw Venn Diagram website, we can obtain the related target of EAA decoction acting on bradyarrhythmia.Then, DAVID database was used to analyze the functional enrichment of gene bulk(GO) and the enrichment of KEGG pathway.The characteristics of network targets are analyzed by using Cytoscape,and the system molecular docking is carried out by using systemsdock database. Result: We screened out 51 active components and their action targets, 566 targets related to bradyarrhythmia, 14 targets of intersection. 63 related biological processes and6 molecular functions were obtained by GO analysis. 10 cell types;Five signal pathways were obtained by enrichment and screening of KEGG pathway. There is a good binding activity between the molecules and the target. Conclusion EAA decoction may play a role in the treatment of bradyarrhythmia through the salivary secretion,salivary secretion,cGMP-PKG signaling pathway,calcium signaling pathway, cAMP signaling pathway.
作者
罗蔚
郑景辉
徐文华
伍新诚
李建橡
Luo Wei;Zheng Jinghui;Xv Wenhua;Wu Xincheng;Li Jianxiang(Ruikang Hospital affiliated to Guangxi University of traditional Chinese Medicine,Nanning Guangxi,532200;Graduate School of Guangxi University of traditional Chinese Medicine,532200)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2019年第11期2338-2346,共9页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委员会地区科学基金项目(81660776):基于线粒体脂质-蛋白质组学的养心通脉有效部位方抗大鼠心肌缺血再灌注损伤机制的研究,负责人:郑景辉
广西自然科学基金委员会面上项目(2016JJA140115):养心通脉有效部位方干预缺血再灌注损伤心肌细胞线粒体的脂质组学研究,负责人:郑景辉
广西科学研究与技术开发计划项目科技攻关计划(桂科攻1598012-55):中医药干预干细胞治疗缺血性心肌病转化医学平台建设与应用,负责人:郑景辉。
关键词
麻黄细辛附子汤
缓慢型心律失常
系统药理学
信号通路
Ephedra Asarum Aconite(EAA)decoction
bradyarrhythmia
systematic pharmacology
signal pathway
