摘要
目的利用系统药理学方法探究冠心Ⅱ号方治疗冠心病的作用机制。方法利用TCMSP数据库筛选出冠心Ⅱ号方五味药所含有效化学成分,用DrugBank数据库和PharmMapper服务器对有效化学成分进行作用靶点预测;通过GeneGards和CTD数据库筛选出与冠心病相关靶点,将疾病相关靶点与化合物靶点进行对比得到交集靶点,利用David在线分析工具对交集靶标基因进行GO分析和KEGG分析,用IGEMDOCK软件验证靶标基因与活性成分的结合活性。结果共得出150个活性成分,对应176个靶点,与冠心病相关靶点22个,网络分析结果表明靶点参与血压调节、一氧化氮生物合成过程的正调控、谷胱甘肽代谢过程等生物学过程;线粒体、细胞外间隙、神经元胞体等细胞组成;超氧化物歧化酶活性、蛋白酪氨酸激酶活性等分子功能;调节FOXO信号转导通路、ErbB信号通路、HIF-1信号通路和AMPK信号通路等通路发挥治疗冠心病作用。结论本研究从系统药理学层面初步揭示了冠心Ⅱ号方治疗冠心病的作用机制,冠心Ⅱ号方治疗冠心病具有多成分、多靶点、多途径等特点,为下一步深入验证研究提供了良好的理论基础和新的思路。
Objective to explore the mechanism of Guanxin ⅱprescription in the treatment of coronary heart disease bysystematic pharmacology. Methods the effective chemical constituents of Guanxin Ⅱ prescription were screened by TCMSP database, and the effective chemical constituents were predicted by DrugBank database and Pharmmapper server. GeneGards and CTD databases were used to screen the coronary heart disease related targets, and the disease related targets were compared with the compound targets to obtain the intersection targets. The cross target genes were analyzed by GO and KEGG using David online analysis tool, and the binding activity of target gene to active components was verified by IGEMDOCK software. Results a total of 150 active components were obtained, corresponding to 176 targetsand 22 targets related to coronary heart disease. The results of network analysis showed that the targets were involved in the regulation of blood pressure, the positive regulation of nitric oxide biosynthesis, and the process of glutathione metabolism. Cell composition of mitochondria, extracellular space, neuronal cell body, superoxide dismutase activity, protein tyrosine kinase activity and other molecular functions;Regulation of FOXO signal transduction pathway,ErbB signaling pathway, HIF-1 signal pathway and AMPK signaling pathway play a role in the treatment of coronary heart disease. Conclusion in this study, the treatment of Guanxin Ⅱ prescription was preliminarily revealed from the level of systematic pharmacology. The mechanism of coronary heart disease, Guanxin ⅱ prescription for coronary heart disease has the characteristics of multi-component, multi-target and multi-way, which provides a good theoretical basis and new ideas for further research.
作者
杨文娜
徐文华
徐香梅
陈旋
郑景辉
Yang Wenna;Xu Wenhua;Xu Xiangmei;Chen Xuan;Zheng Jinghui(Guangxi University of traditional Chinese Medicine,Guangxi Nanning 530200;Ruikang Hospital affiliated to Guangxi University of traditional Chinese Medicine,Guangxi Nanning 530011)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2019年第11期2328-2337,共10页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委员会地区科学基金项目(81660766):基于线粒体脂质-蛋白质组学的养心通脉有效部位方抗大鼠心肌缺血再灌注损伤机制的研究
负责人:郑景辉
国家自然科学基金委员会地区科学基金项目(81360535):养心通脉有效部位方诱导骨髓间充质干细胞分化过程中miRNA调控网络研究
负责人:郑景辉
广西自然科学基金面上项目(2016GXNSFAA380296):养心通脉有效部位方干预缺血再灌注损伤心肌细胞线粒体的脂质组学研究
负责人:郑景辉。
关键词
系统药理学
冠心Ⅱ号方
冠心病
分子对接
信号通路
systematic pharmacology
Guanxin ⅱ prescription
Coronary heart disease
Molecular docking
signal pathway
