摘要
以金黄花滇百合(Lilium bakerianumvar. aureum)的鳞片为外植体,探讨不同部位外植体和不同配比的植物生长调节剂对愈伤组织诱导和植株再生的影响。研究结果表明,鳞片诱导愈伤组织和不定芽的最适培养基为MS+1.0mg·L^-16-BA+0.1 mg·L^-1 NAA+30 g·L^-1蔗糖;不定芽增殖的最适培养基为MS+0.5 mg·L^-1 6-BA+0.1 mg·L^-1 NAA+30 g·L^-1蔗糖;不定芽诱导小鳞茎的最适培养基为MS+1.0 mg·L^-1 NAA+30g·L^-1蔗糖;小鳞茎膨大生根的最适培养基为1/2MS+0.01 mg·L^-1NAA+60 g·L^-1蔗糖。该研究为金黄花滇百合种质资源保护和快速繁殖提供了技术支撑。
We investigated the effects of different explants, kinds and concentrations of plant growth regulators on callus induction and plant regeneration from scales of Lilium bakerianum var. aureum at tissue culture. The medium MS+1.0 mg·L^-1 6-BA+0.1 mg·L^-1 NAA+30 g·L^-1 sucrose was suitable for inducing adventitious buds and calli. The medium MS+0.5 mg·L^-1 6-BA+0.1 mg·L^-1 NAA+30 g·L^-1 sucrose was suitable for adventitious bud multiplication. MS+1.0 mg·L^-1 NAA+30 g·L^-1 sucrose was optimal for bulblet formation from adventitious buds, and the optimal medium for the growth of bulblets was 1/2 MS+0.01 mg·L^-1 NAA+60 g·L^-1 sucrose. We provide technical support for germplasm resource protection and rapid propagation of L. bakerianum var. aureum.
作者
张文婷
何燕红
舒宁
邢景景
刘宝骏
包满珠
刘国锋
Zhang Wenting;He Yanhong;Shu Ning;Xing Jingjing;Liu Baojun;Bao Manzhu;Liu Guofeng(Key Laboratory of Horticultural Plant Biology,Ministry of Education,College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China;Guangzhou Institute of Forestry and Landscape Architecture,Guangzhou 510405,China)
出处
《植物学报》
CAS
CSCD
北大核心
2019年第6期773-778,共6页
Chinese Bulletin of Botany
基金
公益性行业(农业)科研项目(No.201203071)
关键词
金黄花滇百合
组织培养
植株再生
快速繁殖
愈伤组织
Lilium bakerianum var.aureum
tissue culture
plant regeneration
rapid propagation
calli
