摘要
为解决川续断种苗快速繁殖的问题,需建立高效的组培苗再生体系。本研究利用植物组培技术,筛选川续断组培的最佳外植体,运用响应面优化法对川续断愈伤组织及丛生芽的诱导体系进行优化。结果显示,适合川续断组培的最佳外植体为幼苗叶片,最佳愈伤组织诱导培养基:MS+6-BA0.93 mg/L+NAA0.64 mg/L+KT 0.55 mg/L,诱导率98.62%,不同激素对愈伤组织诱导率大小的影响顺序:NAA>6-BA>KT,不同激素组合对愈伤组织诱导率大小的影响顺序为:NAA+KT>NAA+6-BA>6-BA+KT;丛生芽诱导的最佳培养基:MS+6-BA 0.76 mg/L+GA33.51 mg/L+IAA 0.93 mg/L,诱导率88.49%,不同激素对丛生芽诱导率大小的影响顺序为:GA3>6-BA>IAA,不同激素组合对丛生芽诱导率大小的影响顺序为:GA3+6-BA>GA3+IAA>6-BA+IAA;生根壮苗的最佳培养基:1/4MS+6-BA 0.1 mg/L+GA30.5 mg/L+0.5%活性炭,生根率81.92%。本研究建立的川续断组培苗高效再生体系,可为下一步研发川续断快繁优质种苗提供关键技术资料。
In order to solve the problem of rapid propagation of Dipsacus asperoides seedlings,an efficient regeneration system of tissue culture was established.In this study,plant tissue culture technology was used to select the best explants,and response surface optimization method was used to optimize the induction system of callus and cluster bud of D.asperoides.The results showed that the best explant for tissue culture of D.asperoides was sterile leaves,and the best medium for callus induction was MS+6-BA 0.93 mg/L+NAA 0.64 mg/L+KT 0.55 mg/L,the induction rate was 98.62%.The order of different hormones on the induction rate of callus:NAA>6-BA>KT,the order of different hormone combinations on the induction rate of callus:NAA+KT>NAA+6-BA>6-BA+KT;The best medium for cluster bud induction:MS+6-BA 0.76 mg/L+GA33.51 mg/L+IAA 0.93 mg/L,the induction rate was 88.49%.The order of different hormones on the induction rate of cluster buds was GA3>6-BA>IAA.The order of different hormone combinations on the induction rate of cluster buds was GA3+6-BA>GA3+IAA>6-BA+IAA.The best medium for rooting is 1/4MS+6-BA 0.1 mg/L+GA30.5 mg/L+0.5%AC,the rooting rate was 81.92%.The high efficient regeneration system of tissue culture seedling established in this study can provide the key technical data for the next step to solve the problem of D.asperoides rapid propagation.
作者
晋海军
于花
王海霞
朱姗
张嘉
Jin Haijun;Yu Hua;WangHaixia;Zhu Shan;Zhang Jia(Resource Institute for Chinese&EthnicMateriaMedica,Guizhou University of Traditional ChineseMedicine,Guiyang,550025;School of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang,550025;College of Humanities and Management,Guizhou University of Traditional ChineseMedicine,Guiyang,550025)
出处
《分子植物育种》
CAS
北大核心
2021年第17期5816-5824,共9页
Molecular Plant Breeding
基金
贵州中医药大学2018年大学生创新创业训练计划项目(2018-29)
贵州省国内一流建设学科(中药学)(GNYL〔2017〕008号)
贵阳中医学院博士启动金项目(043160005)
贵阳中医学院2017学术新苗培养及创新探索专项项目(黔科合平台人才〔2017〕5735号-19)共同资助。
