摘要
目的:建立金草消毒颗粒的质量标准。方法:采用TLC法鉴别金草消毒颗粒中3味中药饮片:肿节风、白花蛇舌草、半枝莲;同时以HPLC法测定异嗪皮啶、迷迭香酸含量,色谱柱:Aglient Zorbax SB-C18柱(250 mm×4.6 mm,5μm),流动相:乙腈-0.2%磷酸(25∶75)等度洗脱,流速:1.0 ml·min^-1,检测波长:342 nm,柱温:25℃。结果:薄层色谱斑点清晰,分离度好,阴性无干扰;异嗪皮啶和迷迭香酸分别在5.23~31.39μg·ml^-1(r=0.9984)、2.62~30.10μg·ml^-1(r=0.9997)范围内线性关系良好,平均回收率分别为89.18%(RSD=1.51%)、107.68%(RSD=0.85%)(n=6)。结论:本研究建立的质量控制方法快速、简单、专属性强,适用于金草消毒颗粒的质量控制。
Objective:To establish the quality standard for Jincao Xiaodu granules.Methods:TLC was used for identifying Sarcandra glabra,Hedyotis diffusa Willd and Scutellaria barbata in Jincao Xiaodu granules.The contents of isoxazinidine and rosemic acid were analyzed by high performance liquid chromatography on an Aglient Zorbax SB-C18 column(250 mm×4.6 mm,5μm)with the mobile phase of acetonitrile-0.2%phosphoric acid(25∶75)with isocratic elution at a flow rate of 1.0 ml·min^-1.The detection wavelength was 342 nm and the column temperature was controlled at 25℃.Results:The TLC spots were clear and well-separated that could be distinguished from the negative samples.Isoxazinidine and rosemic acid was linear respectively within the range of 5.23-31.39μg·ml^-1(r=0.9984)and 2.62-30.10μg·ml^-1(r=0.9997),and the average recovery was 89.18%(RSD=1.51%)and 107.68%(RSD=0.85%)(n=6),respectively.Conclusion:The method established in the study is fast,simple and specialized,which can be used for the quality control of Jincao Xiaodu granules.
作者
陈春燕
蒋定之
宋小容
莫迎
付书婕
孔晓龙
蒋伟哲
Chen Chunyan;Jiang Dingzhi;Song Xiaorong;Mo Ying;Fu Shujie;Kong Xiaolong;Jiang Weizhe(College of Pharmacy,Guangxi Medical University,Nanning 530021,China;Guangxi-ASEAN Food and Drug Safety Inspection and Testing Center)
出处
《中国药师》
CAS
2020年第1期176-179,共4页
China Pharmacist
基金
广西创新驱动发展专项-科技重大专项(编号:桂科AA17202050)
广西科技计划重点项目(编号:桂科AB16380187)
广西一流学科(药学)建设项目(编号:GXFCDP-PS-2018)
南宁市科技型中小企业技术创新资金项目(编号:20183108)
广西壮族自治区大学生创新创业训练计划项目(编号:201810598063)
广西医科大学大学生创新创业训练计划项目(编号:2018359)
