摘要
目的探讨前列腺癌患者雄激素受体辅助因子(ARA)54和凋亡蛋白酶活化因子(Apaf)的表达及临床意义。方法选取2015年1月~2018年5月我院收治的76例前列腺癌患者设为前列腺癌组,选取同期36例良性前列增生设为前列腺增生组,采用免疫组化法检测与应用实时定量聚合酶链反应(RT-PCR)(SYBR染色法)技术检测两组前列腺标本中ARA 54与Apaf的表达。采用Gleason评分法对患者临床分期进行评估,运用Spearman相关性分析各临床分期与ARA54、Apaf表达水平的相关性。结果前列腺癌组ARA 54阳性、Apaf阳性表达水平均明显低于前列腺增生组(P<0.05),转化生长因子β(TGF-β)阳性表达水平明显高于前列腺增生组(P<0.05);ARA 54与Apaf阳性表达在病理学分级、临床分期、有无远处转移存在明显差异(P<0.05),其中,病理学分级越低、临床分期越高、有远处转移的前列腺癌患者中ARA 54与Apaf阳性表达水平明显降低(P<0.05);Spearman相关性分析结果提示,ARA 54表达水平与Gleason评分分级呈正相关性(r值分别为0.724、0.742、0.768、0.750、0.784,P<0.001);Apaf表达水平与Gleason评分分级呈正相关性(r值分别为0.549、0.488、0.425、0.460、0.562,P<0.05);TGF-β表达水平与Gleason评分分级呈负相关性(r值分别为-0.674、-0.789、-0.856、-0.724、-0.764,P<0.05)。结论 ARA 54与Apaf表达异常与前列腺癌的发生发展有关,ARA 54与Apaf的低表达可促进癌细胞的增殖与生长,因此,调节前列腺癌患者前列腺细胞中ARA 54与Apaf表达具有重要意义。
Objective To investigate the expression and clinical significance of androgen receptor associated factor(ARA) 54 and apoptotic protease activating factor(Apaf) in patients with prostate cancer. Methods 76 prostate cancer patients admitted to our hospital from January 2015 to may 2018 were selected as prostate cancer group, and 36 cases of benign prostatic hyperplasia in the same period were selected as prostate hyperplasia group. The expression of ARA 54 and Apaf were detected by immunohistochemistry and real-time quantitative polymerase chain reaction(RT PCR)(SYBR staining). Gleason scoring method was used to evaluate the clinical stages of patients. Spearman correlation analysis was used to analyze the correlation between the clinical stages and the expression level of ara54 and Apaf. Results The positive levels of ARA 54 and Apaf in prostate cancer group were significantly lower than those in BPH group(P<0.05), and the positive levels of TGF β were significantly higher than those in BPH group(P<0.05). There was a significant difference between the positive expression of ARA 54 and Apaf in pathological grade, clinical stage and distant metastasis(P<0.05). The results of Spearman correlation analysis showed that there was a positive correlation between the expression level of ARA 54 and Gleason score(R values were 0.724, 0.742, 0.768, 0.750, 0.784, P<0.001). The expression level of Apaf was positively correlated with Gleason score(r value was 0.549, 0.488, 0.425, 0.460, 0.562, P<0.05). There was a negative correlation between TGF β expression and Gleason score(r-values were-0.674,-0.789,-0.856,-0.724,-0.764, P<0.05). Conclusion The abnormal expression of ARA 54 and Apaf is related to the occurrence and development of prostate cancer. The low expression of ARA 54 and APAF can promote the proliferation and growth of prostate cancer cells. Therefore, it is of great significance to regulate the expression of Ara 54 and APAF in prostate cancer cells.
作者
陈爽
王强
刘月茹
朱秀波
胡梦雪
CHEN Shuang;WANG Qiang;LIU Yueru;ZHU Xiubo;HU Mengxue(Department of Surgery,Luzhou Traditional Chinese Medicine Hospital,Luzhou 646000,Sichuan,China)
出处
《西部医学》
2020年第1期99-102,106,共5页
Medical Journal of West China
基金
四川省卫生和计划委员会科研课题(2016PJ554)
