摘要
目的探讨淫羊藿素抑制人源性前列腺癌LNCaP细胞珠BALB/c-nu裸小鼠荷瘤组织磷脂酰肌醇3-激酶/蛋白激酶B(P13K/Akt)信号通路及其相关蛋白磷酸化的可能机制。方法将40只雄性BALB/c-nu裸小鼠按随机对照原则均分为4组[对照组、前列腺癌(PCa)组、阳性药组和实验组]并采用前列腺内注射LNCaP细胞株构建PCa动物模型。实验组每日按0.1 mL/10 g尾静脉注射0.8%淫羊藿素,阳性药组按0.1 mL/10 g尾静脉注射P13K/Akt抑制剂LY294002,对照组和PCa组注射等体积生理盐水。注射4周后比较裸小鼠体质量、前列腺瘤体湿重、体积及P13K/Akt信号通路相关蛋白表达。结果与PCa组比较,实验组前列腺瘤体湿重、体积、P13K、 p-Akt、磷酸化雄激素受体(p-AR)、雄激素受体剪接变异体7(AR-V7)和降钙素(Calcitonin)均较模型组降低(P<0.05),而钙黏蛋白E(E-cadherin)升高(P<0.05)。结论淫羊藿素通过调节PI3K/Akt信号通路抑制PCa的进展,且这一作用与Akt及AR磷酸化有关。
Objective To explore the possible mechanism of icariin inhibiting phosphoinositide 3-kinase/protein kinase B(P13K/Akt) signaling pathway and phosphorylation of related proteins in human prostate cancer(LNCaP) BALB/c-nu nude mice.Methods Totally 40 male BALB/c-nu nude mice were randomly divided into 4 groups(control group,prostate cancer group,positive drug group and experimental group) and the human prostate cancer model was established by intraprostate injection of LNCaP.The experimental group was injected 0.8% icariin by 0.1 mL/(10 g·d) tail vein,the positive group was injected 0.1 mL/10 g tail vein P13K/Akt inhibitor LY294002,and control group and the prostate cancer group were injected the same volume of normal saline.The body weight,wet weight,volume and P13K/Akt signaling pathway-related protein expression in nude mice were compared after 4 weeks of injection.Results The wet weight,volume,P13K,P-Akt,P-AR,AR-V7 and Calcitonin of prostate cancer tissues in the experimental group were lower than those in the model group(P<0.05),while E-cadherin was higher(P<0.05).Conclusion Icariin inhibits the progress of LNCaP by regulating PI3 K/Akt signaling pathway,which is related to the phosphorylation of Akt and AR.
作者
宋登鹏
饶红
韩安艳
武福云
陈德森
SONG Deng-peng;RAO Hong;HAN An-yan;WU Fu-yun;CHEN De-sen(Department of Paediatric Pharmacy,Shiyan Taihe Hospital, Hubei University of Medicine,Shiyan,442000,China;Basic Medical College,Hubei University of Medicine,Shiyan 442000,China)
出处
《实验动物与比较医学》
CAS
2018年第6期428-433,共6页
Laboratory Animal and Comparative Medicine
基金
国家自然科学基金项目(81702639)
2018年十堰市科学技术研究与开发项目计划(18Y12)
