摘要
目的研究中国南海侧扁软柳珊瑚Subergorgia suberosa的化学成分。方法采用中压制备色谱、硅胶色谱、sephadex LH-20柱色谱、ODS柱色谱、薄层制备色谱(PTLC)等手段从侧扁软柳珊瑚中的乙醇提取物分离次生代谢产物。利用核磁共振、质谱等波谱技术结合参考文献数据对照方法对单体化合物进行结构鉴定。结果分离得到倍半萜和甾烷类化合物共14个,其结构分别鉴定为subergorgic acid methyl ester(1)、2β-hydroxy subergorgic acid methyl ester(2)、subergorgic acid(3)、suberosenolA(4)、suberosenolB(5)、suberosenone(6)、suberosanone(7)、3β-羟基-5β-孕甾-20-酮(8)、3β-羟基-5α-孕甾-20-酮(9)、3α-羟基-5β-孕甾-20-酮(10)、孕甾-4-烯-3,20-二酮(11)、3β-羟基-5-烯-孕甾-20-酮(12)、胆甾醇(13)、过氧麦角甾醇(14)。结论化合物5和10首次从软柳珊瑚科中分离得到,化合物14首次从侧扁软柳珊瑚S.suberosa中分离得到。
Ojective To investigate the chemical constitutes of Subergorgia suberosa from South China Sea.Methods We carried out the chemical separation of EtOH extract of S.suberosa based on the chromatographic techniques such as medium pressure preparative chromatography,silica,sephadex LH-20,ODS column chromatography,and preparative thin layer chromatography(PTLC).The NMR and MS spectroscopic techniques,together with comparison with literature data,were used for structural elucidation of compounds.Results Fourteen compounds including sesquiterpenes and steroids were isolated from S.suberosa.These compounds were identified as subergorgic acid methyl ester(1),2β-hydroxy subergorgic acid methyl ester(2),subergorgic acid(3),suberosenol A(4),suberosenol B(5),suberosenone(6),suberosanone(7),3β-hydroxy-5β-pregnan-20-one(8),3β-hydroxy-5α-pregnan-20-one(9),3α-hydroxy-5β-pregnan-20-one(10),pregnan-4-ene-3,20-dione(11),3β-hydroxy-pregnan-5-ene-20-one(12),cholesterol(13),and 5α,8α-epidioxyergosta-6,22-dien-3β-ol(14),respectively.Conclusion Compounds 5 and 10 are isolated from the family Subergorgiidae for the first time.Compound 14 is isolated from S.suberosa for the first time.
作者
陈显强
高程海
邢楠楠
杨斌
柴兴云
孙见凡
刘永宏
CHEN Xian-qiang;GAO Cheng-hai;XING Nan-nan;YANG Bin;CHAI Xing-yun;SUN Jian-fan;LIU Yong-hong(Institute of Marine Drugs,Guangxi University of Chinese Medicine,Nanning 530200,China;Key Laboratory of Marine Bio-resources Sustainable Utilization,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China;Guangxi Key Laboratory of Chinese Medicine Foundation Research,Guangxi Scientific Experimental Center of Traditional Chinese Medicine,Guangxi University of Chinese Medicine,Nanning 530200,China)
出处
《中草药》
CAS
CSCD
北大核心
2019年第23期5683-5689,共7页
Chinese Traditional and Herbal Drugs
基金
八桂学者专项资金（05019055）
广西中医药大学岐黄工程高层次人才团队培育项目（2018006）
广西中医药大学海洋药物研究院团队科研专项经费项目（2018ZD005-A01、2018ZD005-A02）
广西高校中青年教师科研基础能力提升项目（2019KY0309）
