摘要
目的探讨细胞型朊蛋白(PrP^C)对口腔癌生长及侵袭迁移的影响。方法通过shRNA转染技术构建PrPC稳定敲减的CAL27细胞。采用CCK8法、细胞粘附实验、划痕实验和侵袭实验检测PrP^C在细胞增殖、粘附、迁移和侵袭中的作用。为进一步确定PrP^C在体内的作用,分别将CAL27和PrP^C敲减CAL27细胞注射至裸鼠背部皮下。定期测量肿瘤体积,采用Ki67免疫组织化学染色和TUNEL法检测移植瘤的细胞增殖和凋亡情况。结果稳定敲减PrP^C明显减弱CAL27细胞活力,降低细胞粘附和侵袭迁移能力。PrP^C敲减明显抑制裸鼠移植瘤生长及细胞增殖,促进肿瘤细胞凋亡。结论PrP^C促进口腔癌生长和侵袭迁移,PrP^C有望成为口腔癌防治新靶点。
Objective To investigate the role of cellular prion protein(PrPC)in the oral cell line CAL27 and xenograft model.Methods A stable reduction in PrPC expression was generated by using shRNA-mediated gene silencing in an OSCC cell line,CAL27 cells.The roles of PrPC were examined in cell proliferation,migration,invasion,and adhesion of CAL27 cells by CCK8,cell adhesion,wound healing and invasion assays.siControl and siPrP CAL27 cells were injected subcutaneously into the nude mice.Tumor volumes were measured,Ki67 antibody and TUNEL staining were used to detect cell proliferation and apoptosis in the tumor xenografts.Results PrPC knockdown significantly reduced cell viabiligy in time-dependent manner in CAL27 cells compared with that in siControl cells.PrPC inhibition decreased the adhesion,migration and invasion of CAL27 cells in vitro.Furthermore,inhibiting PrPC expression in the tumor cells reduced tumor growth and the number of Ki67-positive cells,induced the rates of cell apoptosis in siPrP CAL27 cells implanted tumors.Conclusion PrPC promotes cell proliferation,migration,invasion,adhesion and inhibits cell apoptosis in oral cancer,implicating that PrPC may be a novel target for OSCC prevention and treatment.
作者
王敏
陈慧
葛丽华
杨晶
汤晓飞
WANG Min;CHEN Hui;GE Li-hua;YANG Jing;TANG Xiao-fei(Beijing Institute of Dental Research,Capital Medical University School of Stomatology,Beijing 100050,China)
出处
《北京口腔医学》
CAS
2019年第6期301-305,共5页
Beijing Journal of Stomatology
基金
国家自然科学基金（81470752）
北京市自然科学基金（7152066）
北京口腔医院学科建设基金（15-09-11）
关键词
细胞型朊蛋白
口腔癌
增殖
迁移
侵袭
Cellular prion protein
Oral cancer
Proliferation
Migration
Invasion
